Cao Linlin, Liao Yongde, Su Chengyuan, Tang Linqin, Qi Zhifei, Wei Lixin, Wu Jinyan, Gao Shu
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin, 541004, PR China.
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, 15 Yucai Road, Guilin, 541004, PR China; University Key Laboratory of Karst Ecology and Environmental Change of Guangxi Province (Guangxi Normal University), 15 Yucai Road, Guilin, 541004, PR China.
J Environ Manage. 2022 Jul 1;313:114936. doi: 10.1016/j.jenvman.2022.114936. Epub 2022 Apr 4.
The impact of perfluorooctanoic acid (PFOA) on the anaerobic granular sludge was evaluated through a sequential batch experiment. Results showed that PFOA inhibited the chemical oxygen demand (COD) removal rate of the sludge and the dosage of 100 mg/L PFOA was more obvious. However, this negative effect would gradually weaken with the adaptation of microorganisms. For the 50 mg/L PFOA experimental group, the proteins content in the extracellular polymeric substances (EPS) of the anaerobic granular sludge increased from 1.53 mg/g to 3.65 mg/g. Meanwhile, PFOA inhibited the 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride (INT) activity of the anaerobic granular sludge. Furthermore, 100 mg/L PFOA reduced the relative abundance of Proteobacteria by 5.99% and Longilinea by 1.11%. 100 mg/L PFOA mainly restricted COD removal by affecting the glycolysis process, with the abundances of glucokinase and pyruvate kinase reduced by 8% and 28.1%, respectively. Compared with the control group, the relative abundance of the methyl-coenzyme M reductase alpha subunit increased by 84%, respectively, under 100 mg/L PFOA.
通过序批式实验评估了全氟辛酸（PFOA）对厌氧颗粒污泥的影响。结果表明，PFOA抑制了污泥的化学需氧量（COD）去除率，100 mg/L PFOA的剂量影响更为明显。然而，随着微生物的适应，这种负面影响会逐渐减弱。对于50 mg/L PFOA实验组，厌氧颗粒污泥胞外聚合物（EPS）中的蛋白质含量从1.53 mg/g增加到3.65 mg/g。同时，PFOA抑制了厌氧颗粒污泥的2-（对碘苯基）-3-（对硝基苯基）-5-苯基四氮唑氯化物（INT）活性。此外，100 mg/L PFOA使变形菌门的相对丰度降低了5.99%，长线菌属降低了1.11%。100 mg/L PFOA主要通过影响糖酵解过程限制COD去除，其中葡萄糖激酶和丙酮酸激酶的丰度分别降低了8%和28.1%。与对照组相比，在100 mg/L PFOA条件下，甲基辅酶M还原酶α亚基的相对丰度分别增加了84%。
