Ogura Y, Ito T, Ogura M
Biol Chem Hoppe Seyler. 1986 Oct;367(10):1095-9. doi: 10.1515/bchm3.1986.367.2.1095.
The isolated livers from normal, streptozotocin-diabetic, and insulin-treated diabetic rats were perfused without and with infused 7 alpha-hydroxycholesterol. Biliary bile acids were extracted and analysed by gas chromatography. In each liver group, total bile acid concentration was more than four times greater with infused 7 alpha-hydroxycholesterol than without the sterol. Without infused 7 alpha-hydroxycholesterol, bile acids in the control group were composed mainly of beta-muricholic acid and to a lesser extent of cholic acid. In the diabetic group, the ratio between these two bile acids reversed. The ratio tended to be normalized by treatment with insulin. With infused 7 alpha-hydroxycholesterol, the control group secreted chenodeoxycholic acid at a considerable higher percentage besides major beta-muricholic acid and minor cholic acid. In the diabetic group, the ratio between the latter two bile acids reversed as was the case with the endogenous secretion, while the percentage of chenodeoxycholic acid remained then unchanged. The diminished percentage of beta-muricholic acid in the diabetic group was increased two times by treatment with insulin.
对来自正常大鼠、链脲佐菌素诱导的糖尿病大鼠以及胰岛素治疗的糖尿病大鼠的离体肝脏进行灌注，灌注时分别添加和不添加7α-羟基胆固醇。提取胆汁中的胆汁酸并通过气相色谱法进行分析。在每个肝脏组中，灌注7α-羟基胆固醇时的总胆汁酸浓度比未灌注该固醇时高出四倍多。未灌注7α-羟基胆固醇时，对照组的胆汁酸主要由β-鼠胆酸组成，胆酸含量较少。在糖尿病组中，这两种胆汁酸的比例发生了逆转。胰岛素治疗使该比例趋于正常化。灌注7α-羟基胆固醇时，除了主要的β-鼠胆酸和少量胆酸外，对照组分泌鹅去氧胆酸的比例相当高。在糖尿病组中，后两种胆汁酸的比例与内源性分泌情况一样发生了逆转，而鹅去氧胆酸的比例则保持不变。糖尿病组中β-鼠胆酸比例的降低通过胰岛素治疗增加了两倍。
