Department of Pharmacology, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
College of Postgraduates, Henan University of Chinese Medicine, Zhengzhou 450000, China.
J Healthc Eng. 2022 Mar 29;2022:5498130. doi: 10.1155/2022/5498130. eCollection 2022.
To observe the effect of total glycosides of Xiebai on chronic unpredictable stimuli in rats. Changes of serotonin (5-HT), dopamine (DA), norepinephrine (NE), serum corticosterone (CORT), adrenocorticotropic hormone (ACTH), organ index, and histopathology in brain tissue, to explore the treatment and characteristics of total white glucosides in depression model, were prepared by different stimuli in 21 days.
Depression models were replicated by 21 days of chronic mild stimulation in rats. Finally, the levels of CORT and ACTH in the serum of rats with depression model and the levels of NE, DA, and 5-HT in brain homogenates were determined. The thymus and spleen wet weight of each group were measured, and the organ index was calculated. The thymus and pituitary were observed under the light microscope. Pathological changes of the hypothalamus, adrenal gland, and spleen and behavioral indexes of rats in each group were determined.
The results of the experiment showed that 21 days of chronic unpredictable stimulation significantly improved the behavioral response of the model group, and the levels of corticosterone and adrenocorticotropic hormone in the serum and the indicators in the brain homogenate significantly changed, affecting the relevant tissues. The index changes and the results of the combination can prove that this modeling method can be used for the preparation of depression models. The total white glucoside group can significantly improve the above effects, demonstrating that total glucosides of glucoside have a good therapeutic effect on the rat model of depression.
The chronic unpredictability of the total dose of Xiebai glycosides can significantly improve the levels of NE and DA and the levels of serum CORT and ACTH in the brain homogenate of depressive model rats and improve the immune function of the body.
观察白花总苷对慢性不可预知刺激大鼠的影响。通过 21 天的不同刺激，制备了慢性轻度刺激大鼠 21 天的抑郁模型，观察血清皮质酮（CORT）、促肾上腺皮质激素（ACTH）、器官指数和脑组织病理变化，探讨白花总苷在抑郁模型中的治疗作用和特点。
通过 21 天的慢性轻度刺激制备抑郁模型。最后测定抑郁模型大鼠血清中 CORT 和 ACTH 水平及脑匀浆中 NE、DA、5-HT 水平，测量各组大鼠胸腺和脾脏湿重，计算器官指数，观察光镜下各组大鼠胸腺和垂体。观察各组大鼠下丘脑、肾上腺、脾脏的病理变化及行为学指标。
实验结果表明，21 天慢性不可预知刺激明显改善了模型组的行为反应，血清皮质酮和促肾上腺皮质激素水平及脑匀浆各项指标明显改变，影响相关组织。结合指标变化和结果可以证明，这种造模方法可用于制备抑郁症模型。总苷组能明显改善上述作用，表明苷总苷对抑郁模型大鼠有较好的治疗作用。
白花总苷的慢性不可预知性可明显改善抑郁模型大鼠脑匀浆中 NE、DA 及血清 CORT、ACTH 水平，提高机体免疫功能。
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