Beaulieu J F, Calvert R
Can J Physiol Pharmacol. 1986 Aug;64(8):1137-42. doi: 10.1139/y86-193.
The role of thyroxine and insulin in the regulation of proliferation and differentiation of the immature duodenal epithelium of the fetal mouse was investigated using an organ culture method with a serum-free medium. Thyroxine (10 nM) stimulates specifically the activity of maltase. Insulin (125 mU/mL) remains without effect on the maturation of all hydrolytic functions studied. Each hormone significantly increases the percentages of brush border enzyme activities liberated in the medium and reduces the amount of glucose released in the medium. In the presence of dexamethasone (76 nM) the effect of thyroxine on maltase activity is still observed. Finally, thyroxine and insulin do not modify the labelling index in the duodenal crypts of the explants in the presence or absence of dexamethasone. These findings indicate that thyroxine and insulin can act directly on the development of the fetal mouse duodenum at the end of gestation. Nevertheless, their implication in prenatal development of the gut functions appears to be of minor importance.
采用无血清培养基的器官培养方法，研究了甲状腺素和胰岛素在调节胎鼠未成熟十二指肠上皮细胞增殖和分化中的作用。甲状腺素（10 nM）特异性刺激麦芽糖酶的活性。胰岛素（125 mU/mL）对所研究的所有水解功能的成熟均无影响。每种激素均显著增加培养基中释放的刷状缘酶活性的百分比，并减少培养基中释放的葡萄糖量。在地塞米松（76 nM）存在的情况下，仍可观察到甲状腺素对麦芽糖酶活性的影响。最后，无论有无地塞米松，甲状腺素和胰岛素均不改变外植体十二指肠隐窝中的标记指数。这些发现表明，甲状腺素和胰岛素可在妊娠末期直接作用于胎鼠十二指肠的发育。然而，它们在肠道功能产前发育中的作用似乎不太重要。
