Tejeda Carlos, Villegas Marcela, Steuer Pamela, Iranzo Esperanza C, González Nicolas, Ramirez-Reveco Alfredo, Salgado Miguel
Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile; Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
Vet Microbiol. 2022 May;268:109412. doi: 10.1016/j.vetmic.2022.109412. Epub 2022 Mar 30.
Copper and its alloys are natural and very well-proven antimicrobial materials. The mechanisms of action through which copper is highly effective have been described at the molecular and cellular level. However, both the design of the studies carried out and the nature of the microorganisms studied have meant that this research has been of limited scope. In the present study, we examined the action mechanisms of a copper ion treatment on the integrity of Mycobacterium avium subsp. paratuberculosis (MAP), a highly resistant animal pathogen. The copper ion treatment applied to MAP cells, resulted in nucleic acid degradation and disintegration, increased ROS production and protein alteration. However, the observed susceptibility of MAP to copper-based treatment was dose-dependent. Finally, it had no effect on the integrity of the MAP cell wall. This new evidence about the observed tolerance in the MAP cell wall against the copper ions, may help us to understand how we can improve the proposed copper-based treatment, and finally achieve a totally effective alternative to control MAP in calf´s milk.
铜及其合金是天然且经过充分验证的抗菌材料。铜具有高效抗菌作用的作用机制已在分子和细胞水平上得到描述。然而，所开展研究的设计以及所研究微生物的性质都意味着这项研究的范围有限。在本研究中，我们研究了铜离子处理对副结核分枝杆菌（MAP）完整性的作用机制，MAP是一种具有高度抗性的动物病原体。对MAP细胞进行铜离子处理会导致核酸降解和崩解、活性氧生成增加以及蛋白质改变。然而，观察到的MAP对基于铜的处理的敏感性是剂量依赖性的。最后，它对MAP细胞壁的完整性没有影响。关于在MAP细胞壁中观察到的对铜离子的耐受性的这一新证据，可能有助于我们理解如何改进所提出的基于铜的处理方法，并最终实现一种完全有效的替代方法来控制犊牛奶中的MAP。
