Tejeda Carlos, Steuer Pamela, Villegas Marcela, Reyes-Jara Angelica, Iranzo Esperanza C, Umaña Reydoret, Salgado Miguel
Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
Pathogens. 2022 Feb 19;11(2):272. doi: 10.3390/pathogens11020272.
Scientific evidence is scarce for the antimicrobial effect of copper on bacteria characterized as more resistant. Using subsp. (MAP), a highly resistant microorganism, as a pathogen model, copper ion treatment has shown a significant bactericidal effect; however, the sustainability of MAP against copper toxicity was also reported in several studies. Accordingly, the present study aimed to evaluate the impacts of copper on MAP.
This study considered physicochemical properties and copper concentration in a buffer since it could modulate MAP response during the application of copper treatment.
Despite the efficacy of copper ions in significantly reducing the MAP load in Phosphate Buffered Saline, some MAP cells were able to survive. The copper concentration generated by the copper ion treatment device increased significantly with increasing exposure times. MAP bacterial load decreased significantly when treated with copper ions as the exposure times increased. An increase in pH decreased oxygen consumption, and an increase in conductivity was reported after treatment application.
Even with higher concentrations of copper, the efficacy of MAP control was not complete. The concentration of copper must be a key element in achieving control of highly resistant microorganisms.
关于铜对具有更强耐药性的细菌的抗菌作用，科学证据稀少。使用高度耐药的微生物副结核分枝杆菌（MAP）作为病原体模型，铜离子处理已显示出显著的杀菌效果；然而，多项研究也报道了MAP对铜毒性的耐受性。因此，本研究旨在评估铜对MAP的影响。
本研究考虑了缓冲液中的物理化学性质和铜浓度，因为它们可能会在铜处理过程中调节MAP的反应。
尽管铜离子在显著降低磷酸盐缓冲盐水中的MAP数量方面有效，但仍有一些MAP细胞能够存活。随着暴露时间的增加，铜离子处理装置产生的铜浓度显著增加。随着暴露时间的增加，用铜离子处理时MAP细菌数量显著减少。处理后pH值升高，耗氧量降低，电导率升高。
即使铜浓度较高，对MAP的控制效果也不完全。铜浓度必须是实现对高度耐药微生物控制的关键因素。
