Tejeda Carlos, Steuer Pamela, Villegas Marcela, Ulloa Fernando, Hernández-Agudelo José M, Salgado Miguel
Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
Escuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, Chile.
Microorganisms. 2023 Mar 30;11(4):898. doi: 10.3390/microorganisms11040898.
Bacteria are capable of responding to various stressors, something which has been essential for their adaptation, evolution, and colonization of a wide range of environments. Of the many stressors affecting bacteria, we can highlight heavy metals, and amongst these, copper stands out for its great antibacterial capacity. Using () as a model, the action of proteins involved in copper homeostasis has been put forward as an explanation for the tolerance or adaptive response of this mycobacteria to the toxic action of copper. Therefore, the aim of this study was to confirm the presence and evaluate the expression of genes involved in copper homeostasis at the transcriptional level after challenging subsp. (MAP) with copper ions.
Buffer inoculated with MAP was treated with two stressors, the presence of copper homeostasis genes was confirmed by bioinformatics and genomic analysis, and the response of these genes to the stressors was evaluated by gene expression analysis, using qPCR and the comparative ΔΔCt method.
Through bioinformatics and genomic analysis, we found that copper homeostasis genes were present in the MAP genome and were overexpressed when treated with copper ions, which was not the case with HO treatment.
These results suggest that genes in MAP that code for proteins involved in copper homeostasis trigger an adaptive response to copper ions.
细菌能够对各种应激源作出反应，这对它们适应、进化以及在广泛环境中定殖至关重要。在影响细菌的众多应激源中，我们可以突出重金属，其中铜因其强大的抗菌能力而格外突出。以（）为模型，参与铜稳态的蛋白质的作用已被提出，作为这种分枝杆菌对铜的毒性作用产生耐受性或适应性反应的一种解释。因此，本研究的目的是在用铜离子挑战亚种（MAP）后，在转录水平上确认参与铜稳态的基因的存在并评估其表达。
用MAP接种的缓冲液用两种应激源处理，通过生物信息学和基因组分析确认铜稳态基因的存在，并使用qPCR和比较ΔΔCt方法通过基因表达分析评估这些基因对应激源的反应。
通过生物信息学和基因组分析，我们发现铜稳态基因存在于MAP基因组中，并且在用铜离子处理时会过度表达，而HO处理则不然。
这些结果表明，MAP中编码参与铜稳态的蛋白质的基因会引发对铜离子的适应性反应。
