Ma Zhongchen, Deng Xiaoyu, Li Ruirui, Hu Ruirui, Miao Yuhe, Xu Yimei, Zheng Wei, Yi Jihai, Wang Zhen, Wang Yong, Chen Chuangfu
International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University, 832003 Shihezi, Xinjiang, China; Collaborative Innovation Center for Prevention and Control of High Incidence Zoonotic Infectious Diseases in Western China, College of Animal Science and Technology, Shihezi University, 832003 Shihezi, Xinjiang, China.
College of Life Sciences, Shihezi University, 832003 Shihezi, Xinjiang, China.
Vet Microbiol. 2022 May;268:109414. doi: 10.1016/j.vetmic.2022.109414. Epub 2022 Mar 30.
The secretory proteins of Brucella mediate the expression of the bacterium in the host, thereby facilitating intracellular parasitism. With the exception of the recently reported BspJ, the Brucella nucleomodulin has not yet been characterized. We defined the Brucella nucleomodulin BspG and verified six proteins (PCBP1, KMT5C, NDUFS6, PCNA, CIAO2B, and SDHB) that interacted with BspG using a yeast two-hybrid assay and co-immunoprecipitation (CO-IP) screening. The deletion of BspG decreased the intracellular proliferation of B. abortus in both in vivo and in vitro experiments. The analysis found that these interacting proteins were related to energy generation, gene expression, and apoptosis of host cells. The crosstalk between B. abortus nucleomodulin BspG and host DNA replication/mitochondrial respiratory pathways promotes anti-apoptosis and infection, but the mechanism needs additional study.
布鲁氏菌的分泌蛋白介导该细菌在宿主体内的表达，从而促进细胞内寄生。除了最近报道的BspJ外，布鲁氏菌的核调节蛋白尚未得到鉴定。我们确定了布鲁氏菌核调节蛋白BspG，并使用酵母双杂交试验和免疫共沉淀（CO-IP）筛选验证了六种与BspG相互作用的蛋白质（PCBP1、KMT5C、NDUFS6、PCNA、CIAO2B和SDHB）。在体内和体外实验中，BspG的缺失均降低了流产布鲁氏菌的细胞内增殖。分析发现，这些相互作用的蛋白质与宿主细胞的能量产生、基因表达和凋亡有关。流产布鲁氏菌核调节蛋白BspG与宿主DNA复制/线粒体呼吸途径之间的相互作用促进了抗凋亡和感染，但具体机制仍需进一步研究。
