Gao Ge, Hu Jin, Li Zhen, Xu Qin, Wang Cheng-Shuang, Jia Hui-Min, Zhou Hong, Lin Peng, Zhao Wei-Wei
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Shenzhen Key Laboratory of Special Functional Materials & Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
Biosens Bioelectron. 2022 Aug 1;209:114224. doi: 10.1016/j.bios.2022.114224. Epub 2022 Mar 26.
Photon-enabled bioelectronics has long been pursued in modern electronics due to their non-contact, remote-control, and even self-powered function interfacing the biological world with semiconductor devices. The debuting organic photoelectrochemical transistor (OPECT) relies on the photovoltage generated by the semiconductors to modulate the channel conductance, which enables light-fueled operation at zero gate bias. Inspired by the insulating nature of macrobiomolecules and surface capacitance mechanism, herein we demonstrate the biological regulation of the surface capacitance towards new OPECT biodetection, which was exemplified by a CdS quantum dots/TiO nanotubes photoanode accommodating hybridization chain reaction (HCR) amplification with the target of biomarker miRNA-17. Formation of the non-conducting DNA layer from the miRNA-17-oriented HCR could decrease the surface capacitance and increase the corresponding fractional potential drop, shifting the transfer curve horizontally to higher gate voltage and thus producing different drain currents. The OPECT biosensor exhibited a linear relationship with the miRNA-17 concentration on the logarithmic axis in the range from 1 pm. to 10 μm with a detection limit of 1 pm. This work not only represented a generic methodology of miRNA detection, but also provided a universal mechanism for the operation of advanced OPECT bioanalytics.
由于光子驱动的生物电子学具有非接触、远程控制甚至自供电功能，能够将生物世界与半导体器件连接起来，因此在现代电子学中一直备受关注。新型有机光电化学晶体管（OPECT）依靠半导体产生的光电压来调制沟道电导，从而实现零栅极偏置下的光驱动操作。受大分子生物绝缘性质和表面电容机制的启发，在此我们展示了表面电容对新型OPECT生物检测的生物调节作用，以CdS量子点/TiO纳米管光阳极为例，其容纳了以生物标志物miRNA-17为目标的杂交链式反应（HCR）扩增。由面向miRNA-17的HCR形成的非导电DNA层可降低表面电容并增加相应的分数电位降，使转移曲线水平移向更高的栅极电压，从而产生不同的漏极电流。该OPECT生物传感器在对数轴上与miRNA-17浓度在1皮摩尔至10微摩尔范围内呈线性关系，检测限为1皮摩尔。这项工作不仅代表了一种通用的miRNA检测方法，还为先进的OPECT生物分析操作提供了一种通用机制。
