School of Biochemical Engineering, Chaoyang Normal College, Chaoyang 122000, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Anal Chem. 2023 Jul 4;95(26):9983-9989. doi: 10.1021/acs.analchem.3c01185. Epub 2023 Jun 20.
Organic photoelectrochemical transistor (OPECT) bioanalysis has recently emerged as a promising avenue for biomolecular sensing, providing insight into the next-generation of photoelectrochemical biosensing and organic bioelectronics. Herein, this work validates the direct enzymatic biocatalytic precipitation (BCP) modulation on a flower-like BiS photosensitive gate for high-efficacy OPECT operation with high transconductance (), which is exemplified by a prostate-specific antigen (PSA)-dependent hybridization chain reaction (HCR) and subsequent alkaline phosphatase (ALP)-enabled BCP reaction toward PSA aptasensing. It has been shown that light illumination could ideally achieve the maximized at zero gate bias, and BCP could efficiently modulate the device's interfacial capacitance and charge-transfer resistance, resulting in a significantly changed channel current (). The as-developed OPECT aptasensor realizes good analysis performance for PSA with a detection limit of 10 fg mL. This work features direct BCP modulation of organic transistors and is expected to stimulate further interest in exploring advanced BCP-interfaced bioelectronics with unknown possibilities.
有机光电化学晶体管 (OPECT) 生物分析最近作为生物分子传感的一种很有前途的方法出现，为下一代光电化学生物传感和有机生物电子学提供了新的思路。在本工作中，通过直接的酶促生物催化沉淀 (BCP) 调制，在花状 BiS 光敏栅极上实现了高效 OPECT 操作，具有高跨导 ()，这是通过前列腺特异性抗原 (PSA) 依赖的杂交链式反应 (HCR) 和随后的碱性磷酸酶 (ALP) 触发的 BCP 反应对 PSA 适体传感进行了例证。结果表明，光照可以理想地实现零栅偏压下的最大 ，BCP 可以有效地调制器件的界面电容和电荷转移电阻，从而导致明显变化的沟道电流 ()。所开发的 OPECT 适体传感器实现了对 PSA 的良好分析性能，检测限低至 10 fg mL。本工作的特点是对有机晶体管进行直接的 BCP 调制，有望激发人们进一步探索具有未知可能性的先进 BCP 界面生物电子学的兴趣。
