Trethewey J, Baxter D, Fisher B V, Sabey G A, Webb F W
Dev Biol Stand. 1986;64:221-6.
The biological control of insulin is vital as it is administered daily to a large proportion of the population during the major part of their lives. The development of the biological assay of insulin will be described with the progressive evolution of methods eventually to dispense with the need for animals. From the quantal Mouse Convulsion Method using around 600 mice per sample to the quantitative Mouse Blood Glucose method using an average of 120 mice per sample in which the fall in the blood glucose levels is used to estimate the potency of insulin by its hypoglycaemic effect. This was followed by the development of the in vitro Radioreceptor Method which is a procedure based on competition between the binding of insulin and [125I]-insulin to cells of the cultured human IM-9 cell line. The production of highly purified insulin by the Pharmaceutical Industry has made possible the recent introduction of new High Performance Liquid Chromatography technology.
胰岛素的生物控制至关重要，因为在大部分生命时间里，很大一部分人口每天都要使用胰岛素。随着方法的逐步发展，最终无需使用动物，胰岛素生物测定法的发展历程将在此进行描述。从每个样本使用约600只小鼠的定量小鼠惊厥法，到每个样本平均使用120只小鼠的定量小鼠血糖法，在该方法中，血糖水平的下降用于通过其降血糖作用来估计胰岛素的效价。随后发展出了体外放射受体法，该方法基于胰岛素与[125I] -胰岛素对培养的人IM - 9细胞系细胞的结合竞争。制药行业生产的高度纯化胰岛素使得最近新型高效液相色谱技术的引入成为可能。
