Keilacker H, Hildebrandt R, Knospe S, Besch W, Ziegler M
Exp Clin Endocrinol. 1986 Aug;87(3):319-25. doi: 10.1055/s-0029-1210561.
For the purpose of monitoring the yield of the insulin extraction procedure from animal pancreas three methods of insulin determination were compared, i.e. the mouse convulsion test, a radioreceptor assay (RRA) on rat fat cells and a radioimmunoassay (RIA) which was especially laid out for high insulin concentrations. In samples containing actually insulin in general all three methods provided comparable results. Notable differences were only found in proinsulin-containing material. Because of its simplicity and high reproducibility as well as the good agreement of its results with those obtained with the other assays, the RIA turned out to be the most suitable assay. On the other hand, the RRA should be useful in detecting molecular differences between the investigated insulin-like preparations and standard insulin.
为监测从动物胰腺提取胰岛素过程的产量，比较了三种胰岛素测定方法，即小鼠惊厥试验、大鼠脂肪细胞放射受体分析（RRA）和专门用于高胰岛素浓度的放射免疫分析（RIA）。在实际含有胰岛素的样品中，一般来说所有三种方法都能提供可比的结果。仅在含胰岛素原的材料中发现了显著差异。由于其操作简单、重复性高以及结果与其他分析方法获得的结果一致性良好，RIA被证明是最合适的分析方法。另一方面，RRA在检测所研究的胰岛素样制剂与标准胰岛素之间的分子差异方面应该是有用的。
