Panten U, Zünkler B J, Scheit S, Kirchhoff K, Lenzen S
Diabetologia. 1986 Sep;29(9):648-54. doi: 10.1007/BF00869265.
The kinetics of insulin secretion and oxygen uptake in response to D-glucose and tolbutamide were compared in mouse pancreatic islets. In addition, the role of decreased ATP as a driving force for secretagogue-induced oxygen consumption was examined. D-glucose (10-30 mmol/l) triggered a biphasic insulin release which always coincided with a monophasic increase in islet oxygen uptake. In the presence of D-glucose (5-30 mmol/l), tolbutamide (3-500 mumol/l) consistently elicited an initial peak of insulin secretion which was followed by a continued decline. Tolbutamide-induced secretory profiles were accompanied by similar respiratory profiles. Oxygen consumption per ng of insulin released during the test phase was higher after elevation of the glucose concentration than after addition of tolbutamide. In conjunction with 5 or 10 mmol/l D-glucose, but not with 15 or 30 mmol/l D-glucose, tolbutamide (30-100 mumol/l) lowered islet ATP content significantly (p less than 0.02). Phosphocreatine was not found in isolated islets, although they contained substantial creatine kinase activity. It is concluded that the driving force for tolbutamide-induced oxygen uptake is a decrease in the phosphorylation potential caused by the work load imposed by stimulation of the secretion process. However, a major proportion of the respiratory response to glucose also results from enhancement of biosynthesis.
在小鼠胰岛中比较了对D-葡萄糖和甲苯磺丁脲反应时胰岛素分泌和氧摄取的动力学。此外，还研究了ATP减少作为促分泌剂诱导氧消耗驱动力的作用。D-葡萄糖（10 - 30 mmol/L）引发双相胰岛素释放，这总是与胰岛氧摄取的单相增加同时发生。在存在D-葡萄糖（5 - 30 mmol/L）的情况下，甲苯磺丁脲（3 - 500 μmol/L）持续引发胰岛素分泌的初始峰值，随后持续下降。甲苯磺丁脲诱导的分泌曲线伴随着类似的呼吸曲线。在测试阶段，葡萄糖浓度升高后每纳克胰岛素释放时的氧消耗高于添加甲苯磺丁脲后。与5或10 mmol/L D-葡萄糖联合使用时，但与15或30 mmol/L D-葡萄糖联合使用时则不然，甲苯磺丁脲（30 - 100 μmol/L）显著降低了胰岛ATP含量（p < 0.02）。在分离的胰岛中未发现磷酸肌酸，尽管它们含有大量的肌酸激酶活性。得出的结论是，甲苯磺丁脲诱导氧摄取的驱动力是由分泌过程刺激所施加的工作负荷导致的磷酸化电位降低。然而，对葡萄糖呼吸反应的很大一部分也是由生物合成增强引起的。
