Hnátková Z, Vraný B, Hnátek J, Lettl A
Folia Microbiol (Praha). 1986;31(5):382-6. doi: 10.1007/BF02936603.
The preparation of toxic cultures of Clostridium septicum is described, using an apparatus with a straight dialysis tubing, where the medium is filled both into the nutrition and cultivation space of the apparatus. Using the cultivation to nutrition volume rate 1:2, mean titre of lethal antigen in filtrates 3.86 limes mortis per mL and 300 dosis lethalis minima per Lm was obtained in comparison with the values of 2.22 and 150 respectively in flask filtrates. Native filtrates of dialyzed cultures were better antigens for hyperimmunization of horses than the culture filtrates from flasks.
本文描述了败血梭菌有毒培养物的制备方法，使用带有直型透析管的装置，将培养基填充到该装置的营养和培养空间中。以1:2的培养体积与营养体积比，滤液中致死抗原的平均效价为每毫升3.86个致死量，每升最小致死剂量为300，而烧瓶滤液中的相应值分别为2.22和150。透析培养物的天然滤液比烧瓶培养滤液更适合用于马匹的超免疫接种。
