Hamaoka T, Terakado N, Nakamura S
Touhoku Branch Laboratory, National Institute of Animal Health, Aomori, Japan.
Microbiol Immunol. 1994;38(2):149-52. doi: 10.1111/j.1348-0421.1994.tb01756.x.
Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of C. septicum, in IFA. In immunoblot analysis with the mAbs, the bands at molecular mass of 35 kDa were found in all C. chauvoei strains, while the bands at 36 kDa were found in 4 of 5 strains of C. septicum. These results indicate that the 35 kDa antigen of C. chauvoei and the 36 kDa antigen of C. septicum possess a similar epitope recognized by the mAb.
通过间接免疫荧光法（IFA）和免疫印迹分析检测了产气荚膜梭菌和败血梭菌常见的乙二胺四乙酸（EDTA）可溶性抗原。使用了对产气荚膜梭菌菌株ATCC 10092的35 kDa抗原具有特异性的单克隆抗体（mAb）。这些单克隆抗体在IFA中与所有11株菌株发生反应，其中6株为产气荚膜梭菌，5株为败血梭菌。在用这些单克隆抗体进行的免疫印迹分析中，在所有产气荚膜梭菌菌株中均发现了分子量为35 kDa的条带，而在5株败血梭菌中的4株中发现了36 kDa的条带。这些结果表明，产气荚膜梭菌的35 kDa抗原和败血梭菌的36 kDa抗原具有一个被该单克隆抗体识别的相似表位。
