Sharma C, Dalferes E R, Radhakrishnamurthy B, Rosen E L, Berenson G S
Inflammation. 1986 Dec;10(4):403-11. doi: 10.1007/BF00915824.
Serum proteins and hemoglobins show increased nonenzymatic glycosylation in diabetes mellitus. The measure of glycosylated proteins, particularly hemoglobin, is considered to be a preferred indicator in the control of diabetes. In a study of diabetes and inflammation, we assessed the extent of nonenzymatic glycosylation of proteins of granulation tissue from diabetic rats. Five, seven, and ten days after carrageenan injection, the granuloma proteins were extracted. Nonenzymatic glycosylation was measured in soluble and insoluble granuloma proteins by thiobarbituric acid assay. Protease activities and free amino groups were assayed in soluble extracts. Nonenzymatic glycosylation in soluble proteins of both groups reached a maximum on the seventh day. However, nonenzymatic glycosylation in soluble proteins of the diabetic granulomas was significantly greater than the controls on days five and seven. During the days after granuloma induction, nonenzymatic glycosylation in the insoluble granuloma tissue proteins gradually decreased without any significant differences between controls and diabetics. Significant decreases in the free amino groups in soluble proteins of the diabetic tissues were noted. Greater activities of cathepsins B and D were noted in diabetic tissues over controls. These observations suggest that, in addition to increased proteolysis, increased nonenzymatic glycosylation of tissue proteins could be associated with the impaired process of wound healing in diabetics.
血清蛋白和血红蛋白在糖尿病中显示出非酶糖基化增加。糖基化蛋白，尤其是血红蛋白的测量，被认为是糖尿病控制中的首选指标。在一项关于糖尿病与炎症的研究中，我们评估了糖尿病大鼠肉芽组织蛋白的非酶糖基化程度。在注射角叉菜胶后5天、7天和10天，提取肉芽肿蛋白。通过硫代巴比妥酸测定法测量可溶性和不溶性肉芽肿蛋白中的非酶糖基化。在可溶性提取物中测定蛋白酶活性和游离氨基。两组可溶性蛋白中的非酶糖基化在第7天达到最大值。然而，在第5天和第7天，糖尿病肉芽肿可溶性蛋白中的非酶糖基化明显高于对照组。在肉芽肿诱导后的几天里，不溶性肉芽肿组织蛋白中的非酶糖基化逐渐降低，对照组和糖尿病组之间没有任何显著差异。注意到糖尿病组织可溶性蛋白中的游离氨基显著减少。与对照组相比，糖尿病组织中组织蛋白酶B和D的活性更高。这些观察结果表明，除了蛋白水解增加外，组织蛋白非酶糖基化增加可能与糖尿病患者伤口愈合过程受损有关。
