Vlassara H, Brownlee M, Cerami A
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5190-2. doi: 10.1073/pnas.78.8.5190.
A new affinity chromatography system that selectively retains glycosylated amino acids has been utilized to determine the amount of nonenzymatic glycosylation present in peripheral nerve from diabetic and control rats and dogs. The mean value for glycosylated amino acids in diabetic rats was 2.8 times greater than the mean value in normal rats (P less than 0.001). In diabetic dogs, mean values were 2.15 times greater than normal values (P less than 0.05). Amino acid analysis of reduced, glycosylated amino acids previously isolated by affinity chromatography showed that glycosylated lysine and its hydrolysis rearrangement products were the major borohydride-reducible adduct present. In addition, another glycosylated product was noted to be present in major proportions. This radioactive product did not chromatograph with any of the available glycosylated amino acid standards. The finding that diabetes results in a nearly 3-fold increase of peripheral nerve glycosylation is consistent with a number of previous investigations in which glycosylation was measured in hemoglobin, serum albumin, and urinary amino acids and peptides from diabetics and normals. The results reported here provide evidence that increased nonenzymatic glycosylation is occurring in a tissue where physiological, morphological, and clinical degeneration characteristically develop as a result of diabetes mellitus.
一种能选择性保留糖基化氨基酸的新型亲和色谱系统已被用于测定糖尿病大鼠和犬以及对照大鼠和犬的外周神经中存在的非酶糖基化量。糖尿病大鼠中糖基化氨基酸的平均值比正常大鼠的平均值高2.8倍（P小于0.001）。在糖尿病犬中，平均值比正常值高2.15倍（P小于0.05）。对先前通过亲和色谱分离的还原型糖基化氨基酸进行氨基酸分析表明，糖基化赖氨酸及其水解重排产物是主要的可被硼氢化钠还原的加合物。此外，还发现另一种糖基化产物占主要比例。这种放射性产物在任何可用的糖基化氨基酸标准品中都没有色谱峰。糖尿病导致外周神经糖基化增加近3倍这一发现与之前的一些研究一致，在这些研究中对糖尿病患者和正常人的血红蛋白、血清白蛋白以及尿氨基酸和肽中的糖基化进行了测定。此处报道的结果提供了证据，表明在糖尿病会导致生理、形态和临床退化的组织中发生了非酶糖基化增加。
