Bezus Brenda, Esquivel Juan Carlos Contreras, Cavalitto Sebastián, Cavello Ivana
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI, UNLP; CCT-La Plata, CONICET), Calle 47 y 115, B1900ASH La Plata, Provincia de Buenos Aires, Argentina.
Laboratory of Applied Glycobiotechnology, Academic Group of Food Science and Technology, School of Chemistry, Universidad Autonoma de Coahuila, Unidad Saltillo, Saltillo 2528, Coahuila, Mexico.
Int J Biol Macromol. 2022 Jun 1;209(Pt A):290-298. doi: 10.1016/j.ijbiomac.2022.04.019. Epub 2022 Apr 7.
An Antarctic yeast was cultivated to produce and enzymatic extract with polygalacturonase activity, whose biochemical properties were studied. It assisted pectin extraction from lime pomace at 20 °C. The extract produced by Tausonia pullulans 8E had an optimum temperature of 40 °C and optimum pH of 5.0. At 20 °C, it displayed 54% of relative activity. A good pH-stability and thermostability were observed. Hg and Co inhibited its activity in 40 and 11%, respectively. Pectin was obtained from lime pomace at 20 °C, with 15% of yield after 120 min extraction. Uronic acid (46%) and neutral sugars (53%) were determined. Pectin's molecular weight was estimated in the order of 100,000 g mol. By anion-exchange chromatography, pectin-linked and free neutral sugars were observed. The high-methoxyl pectin was used to prepare low-calorie gels. It was demonstrated that this cold-active enzyme allows enzymatic-assisted pectin extraction at 20 °C.
培养了一种南极酵母以生产具有聚半乳糖醛酸酶活性的酶提取物，并对其生化特性进行了研究。它在20℃下辅助从酸橙果渣中提取果胶。由出芽短梗霉8E产生的提取物的最适温度为40℃，最适pH为5.0。在20℃时，它表现出54%的相对活性。观察到良好的pH稳定性和热稳定性。汞和钴分别抑制其活性40%和11%。在20℃下从酸橙果渣中获得果胶，提取120分钟后产率为15%。测定了糖醛酸（46%）和中性糖（53%）。果胶的分子量估计约为100,000 g/mol。通过阴离子交换色谱法，观察到与果胶结合的和游离的中性糖。高甲氧基果胶用于制备低热量凝胶。结果表明，这种冷活性酶能够在20℃下实现酶辅助果胶提取。
