Zhang Chengshou, Guo Quanshi, Tong Zongrui, Chen Silong, Mao Zhengwei, Yu Yibo
Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310020, PR China.
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, PR China.
J Colloid Interface Sci. 2022 Aug;619:348-358. doi: 10.1016/j.jcis.2022.03.132. Epub 2022 Apr 2.
Due to development of surgical techniques and intraocular lens (IOL) implants, vision can often be restored in cataracts patients. However, posterior capsular opacification (PCO) has become the most common and challenging complication in cataracts surgery. While various approaches such as surface modification and drug prophylaxis have been investigated to prevent PCO development, there is no standard treatment that is sufficiently safe and effective to meet clinical demands. Near-infrared (NIR) light-triggered photothermal therapy is an attractive noninvasive treatment for PCO prophylaxis. We fabricated a new type of IOL with excellent biocompatibility, stability, and photothermal conversion property. Polyethyleneimine (PEI) and graphene oxide (GO) were layer-by-layer assembled on model polymethylmethacrylate and IOL substrates, and the thickness, surface roughness, and wettability of the substrates with different numbers of bilayers were evaluated. After the reduction of GO to reduced GO (rGO), a rGO/PEI multilayer thin film with good stability and photothermal conversion capability was obtained. The rGO/PEI multilayer coating was able to induce apoptosis in lens epithelium cells under 808-nm NIR laser irradiation in vitro. Finally, rGO@IOL was implanted into rabbit eyes, and the biocompatibility and ability to prevent PCO were evaluated for 5 weeks. The rGO@IOL implant exhibited excellent PCO prevention ability with the assistance of NIR irradiation and did not induce obvious pathological effects in surrounding healthy tissues. The rGO@IOL implant with good biocompatibility, good physicochemical stability, and excellent photothermal conversion property shows promise for clinical application in PCO prophylaxis.
由于手术技术和人工晶状体（IOL）植入物的发展，白内障患者的视力通常能够得以恢复。然而，后囊膜混浊（PCO）已成为白内障手术中最常见且具有挑战性的并发症。尽管已经研究了诸如表面改性和药物预防等各种方法来防止PCO的发生，但尚无足够安全有效的标准治疗方法来满足临床需求。近红外（NIR）光触发的光热疗法是一种有吸引力的用于预防PCO的非侵入性治疗方法。我们制备了一种具有优异生物相容性、稳定性和光热转换性能的新型IOL。将聚乙烯亚胺（PEI）和氧化石墨烯（GO）逐层组装在模型聚甲基丙烯酸甲酯和IOL基材上，并评估了不同双层数基材的厚度、表面粗糙度和润湿性。将GO还原为还原氧化石墨烯（rGO）后，获得了具有良好稳定性和光热转换能力的rGO/PEI多层薄膜。在体外808 nm NIR激光照射下，rGO/PEI多层涂层能够诱导晶状体上皮细胞凋亡。最后，将rGO@IOL植入兔眼，并在5周内评估其生物相容性和预防PCO的能力。rGO@IOL植入物在NIR照射的辅助下表现出优异的预防PCO的能力，并且在周围健康组织中未诱导明显的病理效应。具有良好生物相容性、良好物理化学稳定性和优异光热转换性能的rGO@IOL植入物在PCO预防的临床应用中显示出前景。
