LINC00511 通过上调 miR-195-5p 来增强 LUAD 的恶性程度。
LINC00511 enhances LUAD malignancy by upregulating GCNT3 via miR-195-5p.
机构信息
Department of Radiology, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu, 610072, Sichuan, China.
Department of Thoracic Surgery, Sichuan Cancer Hospital&Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610041, Sichuan, China.
出版信息
BMC Cancer. 2022 Apr 10;22(1):389. doi: 10.1186/s12885-022-09459-7.
BACKGROUND
Accumulating evidence suggests that LINC00511 acts as an oncogenic long non-coding RNA (lncRNA) in various cancers, including lung adenocarcinoma (LUAD). Hence, we attempted to elucidate the potential role of LINC00511 in LUAD.
METHODS
LINC00511, miR-195-5p, and GCNT3 expression in LUAD was detected by qRT-PCR. Changes in the proliferation, migration, and invasion of LUAD cells after abnormal regulation of LINC00511, miR-195-5p, or GCNT3 were detected by CCK-8, BrdU, wound healing, and transwell assays. Bax and Bcl-2 protein expression was measured by western blotting. Additionally, we identified the targeting effects of LINC00511, miR-195-5p, and GCNT3 using luciferase and RNA immunoprecipitation (RIP) assays.
RESULTS
LINC00511 and GCNT3 were found to be upregulated in LUAD, while miR-195-5p was downregulated. Silencing LINC00511 or GCNT3 decreased the proliferation, migration, invasion, and Bcl-2 protein content in LUAD cells and increased the expression of Bax. Interference with miR-195-5p promoted malignant proliferation of cancer cells. miR-195-5p expression was affected by LINC00511and targeted GCNT3.
CONCLUSION
Silencing LINC00511 promotes GCNT3 expression by inhibiting miR-195-5p and ultimately stimulates the malignant progression of LUAD.
背景
越来越多的证据表明，LINC00511 在多种癌症中作为致癌的长链非编码 RNA（lncRNA）起作用，包括肺腺癌（LUAD）。因此，我们试图阐明 LINC00511 在 LUAD 中的潜在作用。
方法
通过 qRT-PCR 检测 LUAD 中 LINC00511、miR-195-5p 和 GCNT3 的表达。通过 CCK-8、BrdU、划痕愈合和 Transwell 测定检测 LINC00511、miR-195-5p 或 GCNT3 异常调节后 LUAD 细胞增殖、迁移和侵袭的变化。通过 Western blot 测定测定 Bax 和 Bcl-2 蛋白表达。此外，我们使用荧光素酶和 RNA 免疫沉淀（RIP）测定鉴定 LINC00511、miR-195-5p 和 GCNT3 的靶向作用。
结果
发现 LINC00511 和 GCNT3 在 LUAD 中上调，而 miR-195-5p 下调。沉默 LINC00511 或 GCNT3 降低了 LUAD 细胞的增殖、迁移、侵袭和 Bcl-2 蛋白含量，并增加了 Bax 的表达。干扰 miR-195-5p 促进了癌细胞的恶性增殖。miR-195-5p 的表达受 LINC00511 影响，并靶向 GCNT3。
结论
沉默 LINC00511 通过抑制 miR-195-5p 促进 GCNT3 表达，最终刺激 LUAD 的恶性进展。
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