Wei Lianping, Dong Wenhao, Han Zhen, Chen Chen, Jin Qing, He Jinling, Cai Yongping
School of Life Science, Anhui Agricultural University, Hefei, China.
Mabwell (Shanghai) Bioscience Co., Ltd., Shanghai, China.
Front Pharmacol. 2022 Mar 25;13:832134. doi: 10.3389/fphar.2022.832134. eCollection 2022.
Globally, gastric cancer (GC) is one of the three most deadly cancers. () is a traditional Chinese medicine (TCM), and its extract can significantly inhibit the proliferation of gastric cancer cells. However, there are no unified conclusions on its potential active components and possible mechanisms of action. This paper aims at exploring the potential active components, targets, and cell pathways of extract in inhibiting the proliferation of gastric cancer cells by using network pharmacology and cytology experiments. In this paper, UPLC-MS/MS was used to identify the main chemical components in the extracts of , and the an ADME model was used to screen the potential active components. Network pharmacology methods such as target prediction, pathway identification, and network construction were used to determine the mechanism through which the extract inhibited gastric cancer cell proliferation. MTT assays, fluorescence confocal microscopy, clone formation, and flow cytometry were used to verify the inhibitory activity of the extract on gastric cancer cell proliferation . The UPLC-MS/MS analysis identified 178 chemical components from the extract. Network pharmacology analysis showed that 13 chemical components had the potential to inhibit the proliferation of gastric cancer cells, with the possible involvement of 119 targets and 20 potential signaling pathways. experiments confirmed that the extract could significantly inhibit the proliferation of gastric cancer cells. Therefore, we believe that the extract can inhibit the proliferation of gastric cancer cells through effects on multiple components, multiple targets, and multiple pathways.
在全球范围内，胃癌（GC）是三种最致命的癌症之一。（）是一种传统中药（TCM），其提取物能显著抑制胃癌细胞的增殖。然而，关于其潜在活性成分和可能的作用机制尚无统一结论。本文旨在通过网络药理学和细胞学实验探索（）提取物抑制胃癌细胞增殖的潜在活性成分、靶点和细胞途径。本文采用超高效液相色谱-串联质谱（UPLC-MS/MS）鉴定（）提取物中的主要化学成分，并使用ADME模型筛选潜在活性成分。采用靶点预测、通路鉴定和网络构建等网络药理学方法确定（）提取物抑制胃癌细胞增殖的机制。采用MTT法、荧光共聚焦显微镜、克隆形成和流式细胞术验证（）提取物对胃癌细胞增殖的抑制活性。UPLC-MS/MS分析从（）提取物中鉴定出178种化学成分。网络药理学分析表明，13种化学成分具有抑制胃癌细胞增殖的潜力，可能涉及119个靶点和20条潜在信号通路。（）实验证实（）提取物能显著抑制胃癌细胞的增殖。因此，我们认为（）提取物可通过作用于多个成分、多个靶点和多个途径来抑制胃癌细胞的增殖。
