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用于支持返校的新冠病毒污水监测:方法学考量与数据解读

Wastewater surveillance for SARS-CoV-2 to support return to campus: Methodological considerations and data interpretation.

作者信息

Kapoor Vikram, Al-Duroobi Haya, Phan Duc C, Palekar Rakhee S, Blount Bobby, Rambhia Kunal J

机构信息

School of Civil & Environmental Engineering, and Construction Management, University of Texas at San Antonio, San Antonio, TX 78249, USA.

MITRE Corporation, Bedford, MA 01730, USA.

出版信息

Curr Opin Environ Sci Health. 2022 Jun;27:100362. doi: 10.1016/j.coesh.2022.100362. Epub 2022 Apr 2.

DOI:10.1016/j.coesh.2022.100362
PMID:35402756
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8975751/
Abstract

The COVID-19 pandemic has been challenging for various institutions such as school systems due to widespread closures. As schools re-open their campuses to in-person education, there is a need for frequent screening and monitoring of the virus to ensure the safety of students and staff and to limit risk to the surrounding community. Wastewater surveillance (WWS) of SARS-CoV-2 is a rapid and economical approach to determine the extent of COVID-19 in the community. The focus of this review is on the emergence of WWS as a tool for safe return to school campuses, taking into account methodological considerations such as site selection, sample collection and processing, SARS-CoV-2 quantification, and data interpretation. Recently published studies on the implementation of COVID-19 WWS on school and college campuses were reviewed. While there are several logistical and technical challenges, WWS can be used to inform decision-making at the school campus and/or building level.

摘要

由于广泛关闭,新冠疫情对学校系统等各类机构构成了挑战。随着学校重新开放校园进行面授教育,需要对病毒进行频繁筛查和监测,以确保学生和教职员工的安全,并限制对周边社区的风险。对严重急性呼吸综合征冠状病毒2(SARS-CoV-2)的污水监测(WWS)是一种快速且经济的方法,可用于确定社区中新冠疫情的程度。本综述的重点是污水监测作为安全重返校园的工具的出现,同时考虑到诸如地点选择、样本采集与处理、SARS-CoV-2定量以及数据解读等方法学考量。对近期发表的关于在中小学和大学校园实施新冠污水监测的研究进行了综述。虽然存在若干后勤和技术挑战,但污水监测可用于为校园和/或建筑层面的决策提供信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/8975751/49c9d32637b5/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/8975751/5d82b7293a0b/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/8975751/49c9d32637b5/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/8975751/5d82b7293a0b/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da25/8975751/49c9d32637b5/gr2_lrg.jpg

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本文引用的文献

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Show us the data: global COVID-19 wastewater monitoring efforts, equity, and gaps.向我们展示数据:全球新冠病毒废水监测工作、公平性及差距。
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Wastewater Surveillance Can Have a Second Act in COVID-19 Vaccine Distribution.
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污水中病原体和指示病毒浓度的昼夜变化。
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Surveillance of SARS-CoV-2 in sewage from buildings housing residents with different vulnerability levels.对居住在不同脆弱性水平居民的建筑物污水中的 SARS-CoV-2 进行监测。
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废水监测在新冠疫苗分发中可发挥新作用。
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Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach.监测学校人群中 SARS-CoV-2 的发生:基于废水的方法。
PLoS One. 2022 Jun 17;17(6):e0270168. doi: 10.1371/journal.pone.0270168. eCollection 2022.
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Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance.最小化 RT-PCR 检测和定量 SARS-CoV-2 RNA 在废水监测中的误差。
Sci Total Environ. 2022 Jan 20;805:149877. doi: 10.1016/j.scitotenv.2021.149877. Epub 2021 Aug 25.
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JAMA Netw Open. 2021 Sep 1;4(9):e2126447. doi: 10.1001/jamanetworkopen.2021.26447.
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Comparison of approaches to quantify SARS-CoV-2 in wastewater using RT-qPCR: Results and implications from a collaborative inter-laboratory study in Canada.采用 RT-qPCR 定量检测污水中 SARS-CoV-2 的方法比较:来自加拿大合作实验室间研究的结果和意义。
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