infection induces cell apoptosis via multiple pathways revealed by transcriptome analysis.

is a worldwide parasite that can infect almost all kinds of mammals and cause fatal toxoplasmosis in immunocompromised patients. Apoptosis is one of the principal strategies of host cells to clear pathogens and maintain organismal homeostasis, but the mechanism of cell apoptosis induced by remains obscure. To explore the apoptosis influenced by , Vero cells infected or uninfected with the parasite were subjected to apoptosis detection and subsequent dual RNA sequencing (RNA-seq). Using high-throughput Illumina sequencing and bioinformatics analysis, we found that pro-apoptosis genes such as DNA damage-inducible transcript 3 (), growth arrest and DNA damage-inducible α (), caspase-3 (), and high-temperature requirement protease A2 () were upregulated, and anti-apoptosis genes such as poly(adenosine diphosphate (ADP)-ribose) polymerase family member 3 (), B-cell lymphoma 2 (), and baculoviral inhibitor of apoptosis protein (IAP) repeat containing 5 () were downregulated. Besides, tumor necrosis factor (TNF) receptor-associated factor 1 (), , TNF receptor superfamily member 10b (), disabled homolog 2 (DAB2)‍-interacting protein (), and inositol 1,4,5-trisphosphate receptor type 3 () were enriched in the upstream of TNF, TNF-related apoptosis-inducing ligand (TRAIL), and endoplasmic reticulum (ER) stress pathways, and TRAIL-receptor 2 (TRAIL-R2) was regarded as an important membrane receptor influenced by that had not been previously considered. In conclusion, the RH strain could promote and mediate apoptosis through multiple pathways mentioned above in Vero cells. Our findings improve the understanding of the infection process through providing new insights into the related cellular apoptosis mechanisms.