Yilmaz Öztürk Betül, Feyzullazade Narmın, Dağ İlknur, Şengel Tayfun
Central Research Laboratory Application and Research Center, Eskişehir Osmangazi University, Eskişehir, Turkey.
Institute of Science, Biotechnology and Biosafety Department, Eskişehir Osmangazi University, Eskişehir, Turkey.
Microsc Res Tech. 2022 Aug;85(8):2760-2775. doi: 10.1002/jemt.24125. Epub 2022 Apr 11.
Farnesol (trans, trans-3,7,11-trimethyl-2,6,10-dodecatriene-1-ol) is an essential oil component that can be found in a variety of plants. In this study, in vitro effects of farnesol on human lung cancer A549 cell line, colon adenocarcinoma (Caco-2) cell line and healthy human lung epithelial BEAS-2B cell lines, WST-1 cytotoxicity test, dual staining of cell survival (DAPI-PI) analysis, micronucleus test, and transmission electron microscopy (TEM). Farnesol acted in a concentration-dependent manner at the dose ranges studied for cancer cell lines, and while at certain doses it reduced proliferation, interestingly at higher concentrations it induced growth more than the control. In the healthy BEAS-2B cell line, it was tested over a wide range of doses and at all studied concentrations, it did not suppress cellular growth, but rather increased. This seems promising in that farnesol harms cancer cell lines but does not cause significant damage to healthy cells. Obtained TEM data after treatment with farnesol at IC50 dose showed both autophagic and apoptotic findings in cancer cell lines compared to control, and normal findings exhibited in BEAS-2B cell line, cell survival, and micronucleus analyzes showed the presence of apoptotic findings and chromosomal damage as a result of farnesol application in cancer cell lines. RESEARCH HIGHLIGHTS: Farnesol has dose-dependent effects on human lung cancer and colon adenocarcinoma cell lines, with no significant damaging effects on healthy human lung epithelial cell lines. TEM, cell survival, and micronucleus findings support the findings of autophagic, apoptotic, and chromosomal damage on cancer cell lines.
法尼醇（反式，反式-3,7,11-三甲基-2,6,10-十二碳三烯-1-醇）是一种存在于多种植物中的精油成分。在本研究中，探究了法尼醇对人肺癌A549细胞系、结肠腺癌（Caco-2）细胞系和健康人肺上皮BEAS-2B细胞系的体外作用，采用了WST-1细胞毒性试验、细胞存活双重染色（DAPI-PI）分析、微核试验和透射电子显微镜（TEM）。在所研究的癌细胞系剂量范围内，法尼醇呈浓度依赖性作用，在某些剂量下它会降低增殖，但有趣的是，在较高浓度下它比对照诱导生长更多。在健康的BEAS-2B细胞系中，在广泛的剂量范围内进行了测试，在所有研究浓度下，它都没有抑制细胞生长，反而促进了生长。这似乎很有前景，因为法尼醇对癌细胞系有损害，但对健康细胞没有造成显著损伤。用IC50剂量的法尼醇处理后获得的TEM数据显示，与对照相比，癌细胞系中有自噬和凋亡现象，而BEAS-2B细胞系表现正常，细胞存活和微核分析显示，由于法尼醇作用于癌细胞系，出现了凋亡现象和染色体损伤。研究亮点：法尼醇对人肺癌和结肠腺癌细胞系有剂量依赖性作用，对健康人肺上皮细胞系无显著损害作用。TEM、细胞存活和微核结果支持癌细胞系存在自噬、凋亡和染色体损伤的结果。
