Department of Biotechology and Biosafety, Institute of Life Science, Eskisehir Osmangazi University, Eskisehir, Turkey.
Central Research Laboratory Application and Research Center, Eskisehir Osmangazi University, Eskisehir, Turkey.
Int Microbiol. 2021 May;24(2):251-262. doi: 10.1007/s10123-021-00162-4. Epub 2021 Feb 18.
Candida species are opportunistic fungi that can cause mucosal or invasive infections. Especially in biofilm-related infections, resistance is very high to anifungals; therefore more effective treatment strategies are needed. Farnesol(3,7,11-trimethyl-2,6,10-dodecatriene-1-ol) is the quorum sensing (QS) signal molecule and can interact with Candida species both as a QS molecule and as an exogenous agent. The aim of this study was to investigate the effects of farnesol on both the planktonic and biofilm forms of Candida species by colorimetric, microbiological, and electron microscopic methods. Obtained results demonstrated the inhibitory effect of farnesol on the planktonic and biofilm forms of Candida. Farnesol showed a biofilm-enhancing effect at lower concentrations. TEM findings showed the membrane and wall damage, vacuolization, or granulation in cells. SEM images confirmed biofilm reduction in pre-/post-biofilm applications as a result of farnesol treatment. In conclusion, farnesol can be used as an alternative agent to reduce the Candida biofilms, with future studies.
假丝酵母菌属是一种机会性真菌，可引起黏膜或侵袭性感染。特别是在生物膜相关感染中，抗真菌药物的耐药性非常高，因此需要更有效的治疗策略。法尼醇（3,7,11-三甲基-2,6,10-十二碳三烯-1-醇）是群体感应（QS）信号分子，可作为 QS 分子和外源性物质与假丝酵母菌属相互作用。本研究旨在通过比色法、微生物学和电子显微镜方法研究法尼醇对假丝酵母菌属浮游和生物膜形式的影响。研究结果表明，法尼醇对浮游和生物膜形式的假丝酵母菌属均有抑制作用。法尼醇在较低浓度下表现出生物膜增强作用。TEM 结果显示细胞出现膜和壁损伤、空泡化或颗粒化。SEM 图像证实，由于法尼醇处理，生物膜在预/后生物膜应用中减少。总之，法尼醇可用作替代剂来减少假丝酵母菌属生物膜，未来还需进一步研究。
