Akagi Satoshi, Matsukawa Kazutsugu
Division of Dairy Cattle Feeding and Breeding Research, Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
Research and Education Faculty, Kochi University, Nankoku, Japan.
Cell Reprogram. 2022 Jun;24(3):142-149. doi: 10.1089/cell.2022.0003. Epub 2022 Apr 11.
This study examined the relationship between the timing of the first cleavage and development of bovine somatic cell nuclear transfer (SCNT) embryos treated with trichostatin A (TSA). SCNT embryos were visually assessed at 22, 26, and 48 hours after activation. Each embryo with two or more distinct blastomeres was transferred into a microwell and cultured until day 7. Irrespective of TSA treatment, approximately half of the cleaved embryos were observed at 22 hours, and a significantly higher blastocyst formation rate was shown in the SCNT embryos cleaved at 22 hours than those cleaved at ≥26 hours. The blastocyst formation rate of TSA-treated embryos cleaved at 22 hours (80%) was slightly higher than that of the control embryos (70%). In addition, interferon-τ () expression was significantly lower in control SCNT embryos and late-cleaving (>26 hours) TSA-treated embryos than in fertilized (IVF) embryos. However, a significant difference was not observed between TSA-treated SCNT embryos cleaved at 22 and 26 hours, and IVF embryos. These results suggest that TSA treatment has no influence on the timing of the first cleavage of SCNT embryos; however, it slightly improves the blastocyst formation rate and the expression level of in early-cleaving embryos.
本研究检测了用曲古抑菌素A（TSA）处理的牛体细胞核移植（SCNT）胚胎首次卵裂时间与发育之间的关系。在激活后22、26和48小时对SCNT胚胎进行视觉评估。将每个具有两个或更多不同卵裂球的胚胎转移到微孔中并培养至第7天。无论TSA处理如何，约一半的卵裂胚胎在22小时时被观察到，并且在22小时时卵裂的SCNT胚胎中的囊胚形成率显著高于在≥26小时时卵裂的胚胎。在22小时时卵裂的经TSA处理的胚胎的囊胚形成率（80%）略高于对照胚胎（70%）。此外，对照SCNT胚胎和晚期卵裂（>26小时）的经TSA处理的胚胎中的干扰素-τ（）表达显著低于体外受精（IVF）胚胎。然而，在22小时和26小时时卵裂的经TSA处理的SCNT胚胎与IVF胚胎之间未观察到显著差异。这些结果表明，TSA处理对SCNT胚胎的首次卵裂时间没有影响；然而，它略微提高了早期卵裂胚胎的囊胚形成率和的表达水平。
