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延迟囊胚形成通过增加DNA损伤、减少细胞增殖和改变转录因子表达模式降低了猪孤雌生殖囊胚的质量和孵化能力。

Delayed Blastocyst Formation Reduces the Quality and Hatching Ability of Porcine Parthenogenetic Blastocysts by Increasing DNA Damage, Decreasing Cell Proliferation, and Altering Transcription Factor Expression Patterns.

作者信息

Sun Ling, Wang Yan, Yang Mo, Xu Zhuang-Ju, Miao Juan, Bai Ying, Lin Tao

机构信息

School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China.

出版信息

J Dev Biol. 2024 Oct 1;12(4):26. doi: 10.3390/jdb12040026.

DOI:10.3390/jdb12040026
PMID:39449318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11503403/
Abstract

The purpose of this study was to investigate the influence of blastocyst formation timing on the quality of porcine embryos derived from parthenogenetic activation. Newly formed blastocysts at days 6, 7, and 8 of culture [termed formation 6, 7, and 8 blastocysts (F6, F7, and F8 blastocysts)] were obtained, and a series of parameters related to the quality of blastocysts, including apoptosis incidents, DNA replication, pluripotent factors, and blastocyst hatching capacity, were assessed. Delayed blastocyst formation (F7 and/or F8 blastocysts) led to increased levels of ROS, DNA damage, and apoptosis while decreasing the mitochondrial membrane potential, DNA replication, Oct4 levels, and numbers of Sox2-positive cells. F7 blastocysts showed a significantly reduced hatching rate compared to F6 blastocysts; however, F8 blastocysts were unable to develop to the hatching stage. Collectively, our findings suggest a negative correlation between delayed blastocyst formation and blastocyst quality.

摘要

本研究的目的是调查囊胚形成时间对孤雌激活产生的猪胚胎质量的影响。获取培养第6、7和8天新形成的囊胚[称为形成6、7和8囊胚（F6、F7和F8囊胚）]，并评估一系列与囊胚质量相关的参数，包括凋亡事件、DNA复制、多能因子和囊胚孵化能力。囊胚形成延迟（F7和/或F8囊胚）导致活性氧水平、DNA损伤和凋亡增加，同时线粒体膜电位、DNA复制、Oct4水平和Sox2阳性细胞数量降低。与F6囊胚相比，F7囊胚的孵化率显著降低；然而，F8囊胚无法发育到孵化阶段。总体而言，我们的研究结果表明囊胚形成延迟与囊胚质量之间呈负相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/cce92b2d8f60/jdb-12-00026-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/44f292eb6f25/jdb-12-00026-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/87aa0d7747dd/jdb-12-00026-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/35ff00bca105/jdb-12-00026-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/5a764c79c760/jdb-12-00026-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/1137d552a054/jdb-12-00026-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/fe007c601edc/jdb-12-00026-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/ad90a77bec47/jdb-12-00026-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/cce92b2d8f60/jdb-12-00026-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/44f292eb6f25/jdb-12-00026-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/87aa0d7747dd/jdb-12-00026-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/35ff00bca105/jdb-12-00026-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/5a764c79c760/jdb-12-00026-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/1137d552a054/jdb-12-00026-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/fe007c601edc/jdb-12-00026-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/ad90a77bec47/jdb-12-00026-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552d/11503403/cce92b2d8f60/jdb-12-00026-g008.jpg

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本文引用的文献

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Hum Reprod. 2023 Sep 5;38(9):1690-1699. doi: 10.1093/humrep/dead136.
2
Blastocyst Cell Number and Allocation Affect the Developmental Potential and Transcriptome of Bovine Somatic Cell Nuclear Transfer Embryos.囊胚细胞数量和分配影响牛体细胞核移植胚胎的发育潜能和转录组。
Stem Cells Dev. 2023 Sep;32(17-18):515-523. doi: 10.1089/scd.2022.0292. Epub 2023 Jul 18.
3
Oxidative stress-induced apoptosis and autophagy: Balancing the contrary forces in spermatogenesis.
氧化应激诱导的凋亡和自噬：在精子发生中平衡相反的力量。
Biochim Biophys Acta Mol Basis Dis. 2023 Aug;1869(6):166742. doi: 10.1016/j.bbadis.2023.166742. Epub 2023 May 3.
4
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency.在牛胚胎中的碱基编辑揭示了 SOX2 在调控多能性方面的种特异性作用。
PLoS Genet. 2022 Jul 5;18(7):e1010307. doi: 10.1371/journal.pgen.1010307. eCollection 2022 Jul.
5
Effects of Trichostatin A on the Timing of the First Cleavage and Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos.曲古抑菌素A对牛体细胞核移植胚胎首次卵裂时间及发育潜能的影响
Cell Reprogram. 2022 Jun;24(3):142-149. doi: 10.1089/cell.2022.0003. Epub 2022 Apr 11.
6
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Redox Rep. 2022 Dec;27(1):45-52. doi: 10.1080/13510002.2022.2046423.
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DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies.胚胎和配子前的 DNA 损伤：特异性、临床相关性和修复策略。
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