表达 GPR56 的细胞毒性 T 淋巴细胞在活动期类风湿关节炎患者外周血中上调，并反映疾病进展。

Cytotoxic T Lymphocytes Expressing GPR56 are Up-regulated in the Peripheral Blood of Patients with Active Rheumatoid Arthritis and Reflect Disease Progression.

机构信息

Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.

出版信息

Immunol Invest. 2022 Aug;51(6):1804-1819. doi: 10.1080/08820139.2022.2058403. Epub 2022 Apr 11.

DOI:10.1080/08820139.2022.2058403

PMID:35404706

Abstract

OBJECTIVE

This study aims to elucidate the changes in the percentage of GPR56 and/or granzyme B (GZMB) positive cells in rheumatoid arthritis (RA) CD4 and CD8 T lymphocytes, and to explore their clinical value in diagnosing and reflecting the progression of RA.

METHODS

The percentages of GPR56 and/or GZMB positive cells were analyzed in peripheral blood (PB) and spleen T cells in a collagen-induced arthritis (CIA) model established in DBA/1 mice. The percentages of GPR56+ and/or GZMB+ cells were further analyzed in PBs from RA patients and healthy controls. Correlation analysis was performed between clinical indicators and GPR56+, GZMB+, and GPR56+ GZMB+ T cells. Receiver operating characteristic (ROC) curves were used to evaluate the value of GPR56 and GZMB in differentiating active and stable remitting RA.

RESULTS

GPR56+ levels were increased in CD4 and CD8 T cells in the PB of CIA mice. The percentages of GPR56+ and GZMB+ cells were increased in both CD4 and CD8 T cell subsets in patients with active RA. GPR56+, GZMB+, and GPR56+ GZMB+ cells were positively correlated with rheumatoid factor and DAS28. ROC analysis revealed that AUCs for GPR56+, GZMB+, and GPR56+ GZMB+ cell percentages to distinguish active RA from stable remission RA were 0.7106, 0.6941, 0.7024, with cut-off values of 16.35, 16.40, 14.80 in CD4 + T cells, and 0.8031, 0.8086, 0.8196 with cut-off values 60.25, 62.15, 40.15 in CD8 + T cells, respectively.

CONCLUSIONS

GPR56+ and/or GZMB+ T cells are up-regulated in patients with active RA and reflect their condition. The detection of GPR56 and GZMB is helpful for RA disease assessment.

摘要

目的

本研究旨在阐明 GPR56 和/或颗粒酶 B（GZMB）阳性细胞在类风湿关节炎（RA）CD4 和 CD8 T 淋巴细胞中的百分比变化，并探讨其在诊断和反映 RA 进展中的临床价值。

方法

分析胶原诱导关节炎（CIA）模型中 DBA/1 小鼠外周血（PB）和脾脏 T 细胞中 GPR56 和/或 GZMB 阳性细胞的百分比。进一步分析 RA 患者和健康对照者 PB 中 GPR56+和/或 GZMB+细胞的百分比。分析 GPR56+、GZMB+和 GPR56+GZMB+T 细胞与临床指标的相关性。采用受试者工作特征（ROC）曲线评价 GPR56 和 GZMB 在区分活动期和稳定缓解期 RA 中的价值。

结果

CIA 小鼠 PB 中 CD4 和 CD8 T 细胞中 GPR56+水平升高。活动期 RA 患者 CD4 和 CD8 T 细胞亚群中 GPR56+和 GZMB+细胞的百分比均升高。GPR56+、GZMB+和 GPR56+GZMB+细胞与类风湿因子和 DAS28 呈正相关。ROC 分析显示，GPR56+、GZMB+和 GPR56+GZMB+细胞百分比区分活动期 RA 与稳定缓解期 RA 的 AUC 分别为 0.7106、0.6941、0.7024，CD4+T 细胞的截断值为 16.35、16.40、14.80，CD8+T 细胞的截断值为 0.8031、0.8086、0.8196。