Periasamy Vaiyapuri Subbarayan, Athinarayanan Jegan, Alshatwi Ali A
Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Ultrason Sonochem. 2016 Jul;31:449-55. doi: 10.1016/j.ultsonch.2016.01.035. Epub 2016 Jan 29.
Nigella sativa L. (NS) is a plant renowned in traditional holistic medicine systems for almost 1400 years because of its remarkable antioxidant, antimicrobial, anti-inflammatory and anti-cancer properties. The essential oil of N. sativa, in particular, possesses these significant biological properties. However, N. sativa essential oil has many insoluble constituents with properties that have not been fully explored. Nanoemulsion-based insoluble formulations are a widely used carrier system for lipophilic materials. In the present study, we used ultrasonic emulsification, polysorbate 80 and water to formulate a highly stable N. sativa essential oil nanoemulsion (NSEO-NE). To optimize the NSEO-NE preparation, we changed the surfactant concentration, the oil-surfactant mixing ratio and the emulsification time. The droplet size distribution and morphology of the prepared NE was analyzed using dynamic light scattering and scanning electron microscopy, respectively. The droplet size of the NSEO-NE was approximately 20-50 nm in diameter. The anticancer properties of the NE preparation were studied using a modified methyl-thiazolyl-diphenyl tetrazolium bromide (MTT) assay as well as cellular uptake and nuclear morphological analyses. The NSEO-NE significantly reduced the viability of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. The nucleo-cytoplasmic morphological features of NSEO-NE-treated cells included cell membrane blebbing, cytoplasmic vacuolation, marginalization of chromatin, and fragmentation of the nucleus. The results clearly indicate that NSEO-NE induced apoptosis in MCF-7 cells. These findings support the potential application of NSEO-NE in breast cancer therapy, and also merit future translational research.
黑种草（Nigella sativa L.，简称NS）是一种在传统整体医学体系中享有近1400年声誉的植物，因其具有显著的抗氧化、抗菌、抗炎和抗癌特性。特别是黑种草精油具有这些重要的生物学特性。然而，黑种草精油有许多不溶性成分，其特性尚未得到充分探索。基于纳米乳液的不溶性制剂是一种广泛用于亲脂性材料的载体系统。在本研究中，我们使用超声乳化、聚山梨酯80和水配制了一种高度稳定的黑种草精油纳米乳液（NSEO-NE）。为了优化NSEO-NE的制备，我们改变了表面活性剂浓度、油-表面活性剂混合比例和乳化时间。分别使用动态光散射和扫描电子显微镜分析了制备的纳米乳液的液滴大小分布和形态。NSEO-NE的液滴直径约为20-50纳米。使用改良的甲基噻唑基二苯基四氮唑溴盐（MTT）测定法以及细胞摄取和核形态分析研究了纳米乳液制剂的抗癌特性。NSEO-NE显著降低了密歇根癌症基金会-7（MCF-7）乳腺癌细胞的活力。经NSEO-NE处理的细胞的核质形态特征包括细胞膜起泡、细胞质空泡化、染色质边缘化和细胞核碎片化。结果清楚地表明NSEO-NE诱导MCF-7细胞凋亡。这些发现支持了NSEO-NE在乳腺癌治疗中的潜在应用，也值得未来进行转化研究。
