血清蛋白质组学分析揭示了麝香保心丸和速效救心丸对急性心肌梗死大鼠模型的心脏保护作用。
Serum proteomic analysis reveals the cardioprotective effects of Shexiang Baoxin Pill and Suxiao Jiuxin Pill in a rat model of acute myocardial infarction.
作者信息
Song Nixue, Lu Dayun, Wu Gaosong, Wang Shisheng, Zeng Yuanyuan, Zhao Jing, Meng Qian, He Han, Chen Linlin, Zhu Hongwen, Liu Aijun, Li Houkai, Shen Xiaoxu, Zhang Weidong, Zhou Hu
机构信息
CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
出版信息
J Ethnopharmacol. 2022 Jul 15;293:115279. doi: 10.1016/j.jep.2022.115279. Epub 2022 Apr 8.
ETHNOPHARMACOLOGICAL RELEVANCE
Shexiang Baoxin Pill (SBP) and Suxiao Jiuxin Pill (SJP) are traditional Chinese medicines used to treat cardiovascular disease (CVD) in China. However, the mechanism of their therapeutic effect on CVD has not been clearly elucidated yet.
AIMS
The aim of this study is to investigate the cardioprotective effect of SBP and SJP in the treatment of acute myocardial infarction (AMI) model rats by applying serum proteomic approach.
MATERIALS AND METHODS
The rat model of AMI was generated by ligating the left anterior descending coronary artery. 42 rats were randomly divided into four groups: sham-operating (Sham, n = 10) group, model (Mod, n = 8) group, Shexiang Baoxin pills pretreatment (SBP, n = 12) group and Suxiao Jiuxin pills pretreatment (SJP, n = 12) group. Data Independent Acquisition (DIA) proteomic approach was utilized to investigate the serum proteome from the rat individuals. The differentially expressed proteins were subsequently obtained with bioinformatic analysis.
RESULTS
DIA-MS identified 415 proteins within 42 samples, and 84 differentially expressed proteins may contribute to the therapeutic effects of SBP and SJP. GOBP and KEGG pathway analysis of 84 differentially expressed proteins revealed that the proteins were mainly involved in platelet activation and adhesion processes. All 84 differentially expressed proteins presented the same changing tendency in the SBP and SJP groups when compared with the Mod group. Among these 84 proteins, 25 proteins were found to be related to CVD. Among these 25 proteins, ACTB, ACTG1, FGA, FGB, FGG, PF4 and VWF were found to be involved in platelet aggregation and activation. FN1, HSPA5 and YWHAZ were associated with adhesion.
CONCLUSIONS
The results of our study suggest that the cardioprotective effects of SBP and SJP are achieved through the modulation of focal adhesion, platelet activation pathways.
民族药理学相关性
麝香保心丸（SBP）和速效救心丸（SJP）是中国用于治疗心血管疾病（CVD）的传统中药。然而，它们对CVD的治疗作用机制尚未完全阐明。
目的
本研究旨在应用血清蛋白质组学方法探讨SBP和SJP对急性心肌梗死（AMI）模型大鼠的心脏保护作用。
材料与方法
通过结扎左冠状动脉前降支建立大鼠AMI模型。42只大鼠随机分为四组：假手术（Sham，n = 10）组、模型（Mod，n = 8）组、麝香保心丸预处理（SBP，n = 12）组和速效救心丸预处理（SJP，n = 12）组。采用数据独立采集（DIA）蛋白质组学方法研究大鼠个体的血清蛋白质组。随后通过生物信息学分析获得差异表达蛋白。
结果
DIA-MS在42个样本中鉴定出415种蛋白质，84种差异表达蛋白可能与SBP和SJP的治疗作用有关。对84种差异表达蛋白的基因本体生物学过程（GOBP）和京都基因与基因组百科全书（KEGG）通路分析表明，这些蛋白主要参与血小板活化和黏附过程。与模型组相比，SBP组和SJP组中所有84种差异表达蛋白均呈现相同的变化趋势。在这84种蛋白中，发现25种蛋白与CVD相关。在这25种蛋白中，肌动蛋白（ACTB）、γ-肌动蛋白1（ACTG1）、纤维蛋白原α链（FGA）、纤维蛋白原β链（FGB）、纤维蛋白原γ链（FGG）、血小板因子4（PF4）和血管性血友病因子（VWF）参与血小板聚集和活化。纤连蛋白1（FN1）、热休克蛋白家族A成员5（HSPA5）和14-3-3ζ蛋白（YWHAZ）与黏附有关。
结论
我们的研究结果表明，SBP和SJP的心脏保护作用是通过调节黏着斑、血小板活化途径实现的。
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