Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Avda. Francisco Tomás y Valiente, 7, E-28049, Madrid, Spain.
Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Avda. Francisco Tomás y Valiente, 7, E-28049, Madrid, Spain.
Talanta. 2022 Aug 1;245:123459. doi: 10.1016/j.talanta.2022.123459. Epub 2022 Apr 6.
Parabens are chemicals widely used as preservatives in different types of industrial products. In recent years, the concern about the safety of these compounds has increased due to their endocrine disrupting activity. For this reason, their use is highly regulated and even some of them have already been banned. Thus, methods for the sensitive and selective detection of these compounds are required to control their presence in food, cosmetic, and pharmaceutical products. This paper presents an HPLC method with electrochemical detection using disposable screen-printed electrodes (SPE) for simultaneous determination of 6 different parabens in personal care products. Electrochemical behaviour of parabens was studied on SPE with different carbon-based materials as working electrode: carbon, ordered mesoporous carbon and graphene. From these studies, pH, detection potential, and the most adequate SPE were chosen. Due to the wide range of textures and viscosities (e.g., liquid, solid, and semi-solid) of personal care products, adequate sample pretreatments are required before chromatographic measurement. Here, a fast ultrasound-assisted extraction method was applied to simultaneously extract 6 parabens (methyl-, ethyl-, isopropyl-, propyl-, butyl-and benzyl-paraben) from different complex-matrix cosmetic products. Instrumental limits of detection between 20 and 115 μg L were obtained applying +1.0 V (vs. Ag) as detection potential on carbon-based SPE. The total analysis time, including sample extraction and HPLC run, was shorter than 35 min. The proposed method is more versatile and faster than the current available methods and has been successfully applied to determine parabens in commercial samples such as shampoos, body creams, facial tonics, and toothpastes.
对羟基苯甲酸酯是一种广泛应用于各种工业产品的防腐剂。近年来，由于其具有内分泌干扰活性，人们对这些化合物的安全性越来越关注。因此，对它们的使用进行了严格的监管，甚至有些已被禁用。因此，需要开发灵敏和选择性的检测方法来控制这些化合物在食品、化妆品和药品中的存在。本文提出了一种使用电化学检测的 HPLC 方法，采用一次性丝网印刷电极（SPE）同时测定个人护理产品中 6 种不同的对羟基苯甲酸酯。研究了不同碳基材料作为工作电极的对羟基苯甲酸酯在 SPE 上的电化学行为：碳、有序介孔碳和石墨烯。通过这些研究，选择了 pH 值、检测电位和最合适的 SPE。由于个人护理产品的质地和粘度范围很广（例如，液体、固体和半固体），在进行色谱测量之前，需要进行适当的样品预处理。在这里，应用快速超声辅助提取方法同时从不同复杂基质的化妆品产品中提取 6 种对羟基苯甲酸酯（甲基、乙基、异丙基、丙基、丁基和苄基对羟基苯甲酸酯）。在碳基 SPE 上应用 +1.0 V（相对于 Ag）作为检测电位，可获得 20-115μg/L 之间的仪器检测限。包括样品提取和 HPLC 运行在内的总分析时间短于 35 分钟。与现有的方法相比，该方法更加通用和快速，并已成功应用于洗发水、体霜、面部爽肤水和牙膏等商业样品中对羟基苯甲酸酯的测定。
