Department of Molecular Genetics, Institute of Experimental Medicine, Acad. Pavlov Str. 12, 197376 Saint-Petersburg, Russia.
Faculty of Dental Medicine and Medical Technologies, Saint Petersburg State University, 8A 21st Line V.O., 199034 Saint-Petersburg, Russia.
Nutrients. 2022 Mar 27;14(7):1399. doi: 10.3390/nu14071399.
The protective effects of recombinant human lactoferrin rhLF (branded "CAPRABEL™") on the cognitive functions of rat offspring subjected to prenatal hypoxia (7% O, 3 h, 14th day of gestation) have been analyzed. About 90% of rhLF in CAPRABEL was iron-free (apo-LF). Rat dams received several injections of 10 mg of CAPRABEL during either gestation (before and after the hypoxic attack) or lactation. Western blotting revealed the appearance of erythropoietin (EPO) alongside the hypoxia-inducible factors (HIFs) in organ homogenates of apo-rhLF-treated pregnant females, their embryos (but not placentas), and in suckling pups from the dams treated with apo-rhLF during lactation. Apo-rhLF injected to rat dams either during pregnancy or nurturing the pups was able to rescue cognitive deficits caused by prenatal hypoxia and improve various types of memory both in young and adult offspring when tested in the radial maze and by the Novel Object Recognition (NOR) test. The data obtained suggested that the apo-form of human LF injected to female rats during gestation or lactation protects the cognitive functions of their offspring impaired by prenatal hypoxia.
已分析重组人乳铁蛋白 rhLF（商品名为“CAPRABEL™”）对经历产前缺氧（7% O2，3 h，妊娠第 14 天）的大鼠后代认知功能的保护作用。CAPRABEL 中的约 90% rhLF 不含铁（脱铁 LF）。妊娠大鼠在产前（缺氧攻击前后）或哺乳期接受多次 10 mg CAPRABEL 注射。Western blot 显示，在接受脱铁 rhLF 处理的妊娠雌性动物、其胚胎（而非胎盘）和哺乳期接受脱铁 rhLF 处理的哺乳幼崽的器官匀浆中，除了缺氧诱导因子（HIFs）之外，还出现了促红细胞生成素（EPO）。在产前缺氧导致认知缺陷的大鼠中，产前或哺乳期注射给母鼠的脱铁 rhLF 能够恢复认知功能，并在放射迷宫和新颖物体识别（NOR）测试中改善幼年和成年后代的各种类型记忆。获得的数据表明，在妊娠或哺乳期给雌性大鼠注射人 LF 的脱铁形式可以保护其后代因产前缺氧而受损的认知功能。
