Sarwar Muhammad Nauman, Ali Hina Ghulam, Ullah Sana, Yamashita Kentaro, Shahbaz Aiman, Nisar Umair, Hashmi Motahira, Kim Ick-Soo
Nano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan.
Faculty of Inorganic Chemistry, Karlsruhe Institute of Technology, Research Center Helmholtz Institute of Ulm (HIU), 89081 Ulm, Baden-Wurttemberg, Germany.
Polymers (Basel). 2022 Mar 27;14(7):1361. doi: 10.3390/polym14071361.
Antibacterial and cyto-compatible tricomponent composite electrospun nanofibers comprised of polyvinyl alcohol (PVA), copper II oxide nanoparticles (CuONPs), and (bitter gourd, MC) extract were examined for their potential application as an effective wound dressing. Metallic nanoparticles have a wide range of applications in biomedical engineering because of their excellent antibacterial properties; however, metallic NPs have some toxic effects as well. The green synthesis of nanoparticles is undergoing development with the goal of avoiding toxicity. The aim of adding extract was to reduce the toxic effects of copper oxide nanoparticles as well as to impart antioxidant properties to electrospun nanofibers. Weight ratios of PVA and MC extract were kept constant while the concentration of copper oxide was optimized to obtain good antibacterial properties with reduced toxicity. Samples were characterized for their morphological properties, chemical interactions, crystalline structures, elemental analyses, antibacterial activity, cell adhesion, and toxicity. All samples were found to have uniform morphology without any bead formation, while an increase in diameters was observed as the CuO concentration was increased in nanofibers. All samples exhibited antibacterial properties; however, the sample with CuO concentration of 0.6% exhibited better antibacterial activity. It was also observed that nanofibrous mats exhibited excellent cytocompatibility with fibroblast (NIH3T3) cells. The mechanical properties of nanofibers were slightly improved due to the addition of nanoparticles. By considering the excellent results of nanofibrous mats, they can therefore be recommended for wound dressing applications.
对由聚乙烯醇（PVA）、氧化铜纳米颗粒（CuONPs）和苦瓜（MC）提取物组成的具有抗菌性和细胞相容性的三组分复合电纺纳米纤维作为有效伤口敷料的潜在应用进行了研究。金属纳米颗粒因其优异的抗菌性能在生物医学工程中有广泛应用；然而，金属纳米颗粒也有一些毒性作用。纳米颗粒的绿色合成正在发展，目的是避免毒性。添加苦瓜提取物的目的是降低氧化铜纳米颗粒的毒性，并赋予电纺纳米纤维抗氧化性能。保持PVA和苦瓜提取物的重量比恒定，同时优化氧化铜的浓度，以获得具有降低毒性的良好抗菌性能。对样品的形态特性、化学相互作用、晶体结构、元素分析、抗菌活性、细胞粘附和毒性进行了表征。发现所有样品均具有均匀的形态，无任何珠粒形成，而随着纳米纤维中CuO浓度的增加，观察到直径增大。所有样品均表现出抗菌性能；然而，CuO浓度为0.6%的样品表现出更好的抗菌活性。还观察到纳米纤维垫与成纤维细胞（NIH3T3）具有优异的细胞相容性。由于添加了纳米颗粒，纳米纤维的机械性能略有改善。考虑到纳米纤维垫的优异结果，因此可推荐将其用于伤口敷料应用。
