Nano Fusion Technology Research Group, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan.
Department of Applied Biology, Faculty of Textile Science and Technology, Bioresource and Environmental Science, Shinshu University, Tokida 3-15-1, Ueda, Nagano 386-8567, Japan.
Int J Biol Macromol. 2020 Jul 15;155:479-489. doi: 10.1016/j.ijbiomac.2020.03.237. Epub 2020 Mar 30.
Wound dressings are the primary barrier between the wound surface and the outer environment. Here we report the fabrication of cellulose acetate (CA)-Manuka honey (MH) composite nanofibrous mats as a biocompatible and antimicrobial wound dressing. CA mats with different quantities of MH were developed by electrospinning. The ATR-FTIR spectra confirm the inclusion of MH in the composite CA-MH nanofibrous mats. The fibers were continuous and bead-free with acceptable mechanical properties. The fiber diameter increased with an increase in MH content. Inclusion of MH in the electrospun composite CA-MH nanofibrous mats shows high efficacy to prevent bacterial growth on the wound surface. The MH loaded CA nanofiber mats showed good antioxidant abilities, while the ability to free radicalize the DPPH was dependent upon the factors of MH content in the fiber and the time of immersion in the DPPH solution. Besides, the nanofibrous mat's high porosity (85-90%) and WVTR values of 2600 to 1950 g/m/day, suitable for wound breathability and the mats show high cytocompatibility to NIH 3T3 cell line in in vitro testing, proving to be effective for promoting wound healing.
伤口敷料是伤口表面与外环境之间的主要屏障。在这里，我们报告了一种由醋酸纤维素 (CA)-麦卢卡蜂蜜 (MH) 复合纳米纤维垫的制备，作为一种生物相容性和抗菌的伤口敷料。通过静电纺丝制备了具有不同 MH 含量的 CA 垫。ATR-FTIR 光谱证实了 MH 包含在复合 CA-MH 纳米纤维垫中。纤维连续且无珠状，具有可接受的机械性能。纤维直径随 MH 含量的增加而增加。MH 对伤口表面细菌生长的高抑制率，MH 负载 CA 纳米纤维垫具有良好的抗氧化能力，而对 DPPH 的自由基清除能力取决于纤维中 MH 含量和在 DPPH 溶液中浸泡的时间。此外，纳米纤维垫的高孔隙率（85-90%）和 WVTR 值为 2600 至 1950 g/m/天，适合伤口透气性，并且垫在体外测试中对 NIH 3T3 细胞系具有高细胞相容性，证明对促进伤口愈合有效。
