Kariagina A S, Lunin V G, Nikol'skaia I I, Debov S S
Mol Gen Mikrobiol Virusol. 1986 Oct(10):16-21.
The phenotypic characteristics of Shigella sonnei strain 47 containing 7 plasmids of low molecular weight and 2 plasmids 60-100 Md large have been studied. The strains of Escherichia coli containing the single plasmids or plasmid groups from Shigella sonnei have been obtained by transformation and conjugation. The comparison of phenotypes of the obtained strains has helped to find the plasmid location of the determinants for streptomycin resistance (P7), genes for colicinogenicity and colicin immunity (P5), the enzymes of host cell specificity system Sso47I (P6), Sso47II (P4), and the genes for the conjugative DNA transfer (P9). Escherichia coli strains producing individual restriction enzymes SsoI and SsoII have been isolated.
对含有7个低分子量质粒和2个60 - 100兆道尔顿大质粒的宋内志贺氏菌47菌株的表型特征进行了研究。通过转化和接合获得了含有来自宋内志贺氏菌的单个质粒或质粒组的大肠杆菌菌株。对所得菌株表型的比较有助于确定链霉素抗性决定簇(P7)、产大肠杆菌素性和大肠杆菌素免疫基因(P5)、宿主细胞特异性系统Sso47I(P6)、Sso47II(P4)的酶以及接合DNA转移基因(P9)的质粒定位。已分离出产生单个限制酶SsoI和SsoII的大肠杆菌菌株。