Singh R K, Singh A, Misra A K, Khanuja S P, Narula R, Kumar S
Biotechnology Centre, Indian Agricultural Research Institute, New Delhi.
Indian J Exp Biol. 1990 Oct;28(10):994-5.
Wide host range vector plasmids pRK290 and pLAFRI carrying genomic fragments of Rhizobium are transferable both ways between R. meliloti and R. leguminosarum cells on the one hand and to E. coli cells on the other, in triparental matings involving E. coli cells carrying pRK2013, the helper for Tra functions to the vector plasmids. The vector plasmids pRK290 and pLAFRI can be employed for recovering clones harbored by R. leguminosarum and R. meliloti by transfer to Rhizobium cells by direct matings of the library with them.
携带根瘤菌基因组片段的广宿主范围载体质粒pRK290和pLAFRI，一方面可在苜蓿根瘤菌和豌豆根瘤菌细胞之间双向转移，另一方面可在涉及携带pRK2013（载体质粒Tra功能的辅助质粒）的大肠杆菌细胞的三亲本杂交中转移至大肠杆菌细胞。载体质粒pRK290和pLAFRI可用于通过文库与苜蓿根瘤菌和豌豆根瘤菌的直接杂交将其转移至根瘤菌细胞，从而回收它们所携带的克隆。
