Tarasishin L A
Mol Gen Mikrobiol Virusol. 1986 May(5):45-6.
The technique of dot immunoenzyme detection has been elaborated for viral antigens identification. The investigated samples (extracts of infected cells, fractions obtained during viruses and viral proteins purification) are placed in nitrocellulose filters, the free binding sites are blocked and then treated with specific immune serum. The formed antigen-antibody complexes are detected using antispecies immunoglobulins or protein A from Staphylococcus aureus, conjugated with horseradish peroxidase. The brown dots appear at samples location containing the viral antigens. Sensitivity of the technique is 1 ng of protein per sample as tested using adenoviral antigens.
斑点免疫酶检测技术已被用于病毒抗原鉴定。所研究的样本（感染细胞提取物、病毒及病毒蛋白纯化过程中获得的组分）置于硝酸纤维素滤膜上，封闭游离结合位点，然后用特异性免疫血清处理。使用与辣根过氧化物酶偶联的抗种属免疫球蛋白或金黄色葡萄球菌蛋白A检测形成的抗原 - 抗体复合物。在含有病毒抗原的样本位置出现棕色斑点。以腺病毒抗原测试时，该技术的灵敏度为每个样本1 ng蛋白质。
