Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China.
Cells. 2022 Mar 28;11(7):1136. doi: 10.3390/cells11071136.
The influence of harvest time on the photosynthetic protein quality of the red alga was determined using label-free proteomics. Of 2716 differentially abundant proteins that were identified in this study, 478 were upregulated and 374 were downregulated. The top enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) pathways were metabolic processes and biosynthetic pathways such as photosynthesis, light harvesting, and carbon fixation in photosynthetic organisms. Nine important photosynthetic proteins were screened. Correlations among their expression levels were contrasted and verified by western blotting. PSII D1 and 44-kDa protein levels increased with later harvest time and increased light exposure. Specific photoprotective protein expression accelerated growth and development. Biological processes such as photosynthesis and carbon cycling increased carbohydrate metabolism and decreased the total protein content. The results of the present study provide a scientific basis for the optimization of the culture and harvest of .
采用无标记蛋白质组学技术确定了收获时间对红藻光合作用蛋白质量的影响。在本研究中鉴定出的 2716 种差异丰度蛋白中，有 478 种上调，374 种下调。京都基因与基因组百科全书（KEGG）和基因本体论（GO）途径中富集最多的是代谢过程和生物合成途径，如光合作用、光捕获和光合生物中的碳固定。筛选出 9 种重要的光合作用蛋白。通过 Western blot 对比和验证了它们表达水平之间的相关性。PSII D1 和 44 kDa 蛋白水平随着收获时间的推迟和光照的增加而增加。特定的光保护蛋白表达加速了 的生长和发育。光合作用和碳循环等生物过程增加了碳水化合物代谢，降低了总蛋白质含量。本研究结果为 的培养和收获优化提供了科学依据。
