Yang Mingchang, Ma Lizhen, Yang Xianqing, Li Laihao, Chen Shengjun, Qi Bo, Wang Yueqi, Li Chunsheng, Yang Shaoling, Zhao Yongqiang
Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National R&D Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin, China.
Front Nutr. 2022 Jul 7;9:924524. doi: 10.3389/fnut.2022.924524. eCollection 2022.
is an edible red seaweed with high nutritional value. It is widely cultivated and consumed in East Asia and has vast economic benefits. Studies have found that is rich in bioactive substances and is a potential natural resource. In this study, label-free shotgun proteomics was first applied to identify and characterize different harvest proteins in A total of 13,046 different peptides were identified and 419 co-expression target proteins were characterized. Bioinformatics was used to study protein characteristics, functional expression, and interaction of two important functional annotations, amino acid, and carbohydrate metabolism. Potential bioactive peptides, protein structure, and potential ligand conformations were predicted, and the results suggest that bioactive peptides may be utilized as high-quality active fermentation substances and potential targets for drug production. Our research integrated the global protein database, the first time bioinformatic analysis of the proteome during different harvest periods, improves the information database construction and provides a framework for future research based on a comprehensive understanding.
是一种具有高营养价值的可食用红海藻。它在东亚广泛种植和消费，具有巨大的经济效益。研究发现，其富含生物活性物质，是一种潜在的自然资源。在本研究中，首次应用无标记鸟枪法蛋白质组学来鉴定和表征不同收获期的蛋白质。共鉴定出13046种不同的肽，并表征了419种共表达靶蛋白。利用生物信息学研究了蛋白质特性、功能表达以及两种重要功能注释（氨基酸和碳水化合物代谢）的相互作用。预测了潜在的生物活性肽、蛋白质结构和潜在配体构象，结果表明生物活性肽可作为高质量的活性发酵物质和药物生产的潜在靶点。我们的研究整合了全球蛋白质数据库，首次对不同收获期的蛋白质组进行生物信息学分析，改进了信息数据库建设，并基于全面理解为未来研究提供了框架。
