Medical Bioscience Department, Faculty of Natural Sciences, University of the Western Cape, Robert Sobukwe Road, Bellville 7335, South Africa.
Cells. 2022 Apr 2;11(7):1197. doi: 10.3390/cells11071197.
This study aimed to investigate the disruption of cell cycle phases of bEnd.3 cells exposed to cancer paracrine secretion. Cancer cells have been reported to use the secretion of paracrine factors to compromise the endothelial barrier to prepare for their passage into the parenchyma. As cancer cells are known to act differently under conditions of hypoxia, we investigated how conditional media (CM) derived from breast and glioblastoma cells incubated under conditions of normoxia and hypoxia would affect proliferation of brain endothelial cells (bEnd.3).
Brain endothelial cells (bEnd.3) were cultivated with normoxic and hypoxic CM generated from breast cancer MCF7 cells and glioblastoma U-87 cells. Cell proliferation was evaluated using the trypan blue exclusion assay and phases of the cell cycle were evaluated using flow cytometry.
bEnd.3 proliferations was suppressed more aggressively with hypoxic CM after 72 and 96 h; cell cycle analysis showed that paracrine treatment tended to prevent BECs from entering the G2 phase, thus suppressing cell division.
MCF7 and U-87 cells induce suppressed proliferation of BECs deferentially under hypoxia by blocking cell cycle progression to the G2 phase.
本研究旨在探究暴露于癌症旁分泌环境中的 bEnd.3 细胞的细胞周期各时相的改变。据报道，癌细胞利用旁分泌因子的分泌作用来破坏内皮屏障，为其向实质内转移做好准备。由于已知缺氧条件下癌细胞的行为有所不同，我们研究了在常氧和缺氧条件下孵育的乳腺癌 MCF7 细胞和脑胶质瘤 U-87 细胞产生的条件培养基（CM）如何影响脑内皮细胞（bEnd.3）的增殖。
用常氧和缺氧条件下产生的乳腺癌 MCF7 细胞和脑胶质瘤 U-87 细胞的 CM 培养脑内皮细胞（bEnd.3）。用台盼蓝排斥试验评估细胞增殖，用流式细胞术评估细胞周期各时相。
bEnd.3 在 72 和 96 小时后用缺氧 CM 增殖受到更强烈的抑制；细胞周期分析表明，旁分泌处理倾向于阻止 BEC 进入 G2 期，从而抑制细胞分裂。
MCF7 和 U-87 细胞通过阻止细胞周期进入 G2 期，使 BEC 增殖受到不同程度的抑制，从而在缺氧条件下诱导 BEC 增殖。
