Nakano Masataka, Furuichi Takuya, Sokabe Masahiro, Iida Hidetoshi, Yano Sachiko, Tatsumi Hitoshi
Department of Biology, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei-shi 184-8501, Japan.
Research Institute for Science & Technology, Tokyo University of Science, 2641 Yamazaki, Noda-shi 278-8510, Japan.
Plants (Basel). 2022 Mar 31;11(7):956. doi: 10.3390/plants11070956.
The International Space Station (ISS) provides a precious opportunity to study plant growth and development under microgravity (micro-) conditions. In this study, four lines of Arabidopsis seeds (wild type, wild-type MCA1-GFP, -knockout, and -overexpressed) were cultured on a nylon lace mesh placed on Gelrite-solidified MS-medium in the Japanese experiment module KIBO on the ISS, and the entanglement of roots with the mesh was examined under micro- and 1- conditions. We found that root entanglement with the mesh was enhanced, and root coiling was induced under the micro- condition. This behavior was less pronounced in -knockout seedlings, although MCA1-GFP distribution at the root tip of the seedlings was nearly the same in micro--grown seedlings and the ground control seedlings. Possible involvement of MCA1 in the root entanglement is discussed.
国际空间站（ISS）提供了一个在微重力条件下研究植物生长和发育的宝贵机会。在本研究中，将四组拟南芥种子（野生型、野生型MCA1-GFP、基因敲除型和过表达型）培养在放置于国际空间站日本实验舱“希望”号中Gelrite固化的MS培养基上的尼龙花边网片上，并在微重力和1倍重力条件下检查根与网片的缠绕情况。我们发现，在微重力条件下，根与网片的缠绕增强，并且诱导了根的盘绕。尽管在微重力下生长的幼苗和地面对照幼苗根尖处的MCA1-GFP分布几乎相同，但这种行为在基因敲除型幼苗中不太明显。文中讨论了MCA1可能参与根缠绕的情况。
