Nešić Marija, Obratov-Petković Dragica, Skočajić Dragana, Bjedov Ivana, Čule Nevena
Department of Landscape Architecture and Horticulture, Faculty of Forestry, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia.
Department of Environmental Protection and Improvement, Institute of Forestry, Kneza Višeslava 3, 11030 Belgrade, Serbia.
Plants (Basel). 2022 Apr 1;11(7):969. doi: 10.3390/plants11070969.
Invasive species (Willd.) G. L. Nesom is spreading uncontrollably along wet habitats as well as in disturbed ecosystems. All those habitats function as corridors that facilitate seed dispersal. One way to prevent the spread of invasive species is to know their reproductive ecology. The present study evaluates the potential for generative reproduction of and determines how different temperatures, amounts of nutrients, and light regimes, affect seed germination. Seeds collected from 13 natural populations were germinated at four fluctuating temperature regimes (15/6, 20/10, 30/15, and 35/20 °C). To test the influence of nitrate on seed germination, two KNO concentrations were used (5 mM and 50 mM solution). For each treatment, three replicates of 30 seeds were placed in complete darkness or a 14 h photoperiod. The results showed that the germination increased with increasing temperature. The optimal temperature regimes were 30/15 °C and 35/20 °C with approximately 88% germination. The overall effect of KNO on germination was positive. The concentration of 50 mM KNO had a less stimulating effect compared to 5 mM KNO. Seeds showed sensitivity to lack of light during germination but were able to germinate in a significant percentage in such conditions. Considering that often occurs in disturbed sites, these results suggest that seed reaction to alternating temperature, nutrients concentration, and light can be determining factors that affect seed germination of this species and, thus, its spread.
入侵物种（Willd.）G. L. Nesom正在沿着湿地栖息地以及受干扰的生态系统不受控制地蔓延。所有这些栖息地都起到了促进种子传播的走廊作用。防止入侵物种扩散的一种方法是了解它们的繁殖生态学。本研究评估了该物种有性繁殖的潜力，并确定不同温度、养分含量和光照条件如何影响种子萌发。从13个自然种群收集的种子在四种波动温度条件（15/6、20/10、30/15和35/20°C）下进行萌发。为了测试硝酸盐对种子萌发的影响，使用了两种KNO浓度（5 mM和50 mM溶液）。对于每种处理，将30粒种子的三个重复样本置于完全黑暗或14小时光周期条件下。结果表明，发芽率随温度升高而增加。最佳温度条件是30/15°C和35/20°C，发芽率约为88%。KNO对发芽的总体影响是积极的。与5 mM KNO相比，50 mM KNO的刺激作用较小。种子在萌发过程中对光照缺乏敏感，但在这种条件下仍能有相当比例的种子发芽。鉴于该物种经常出现在受干扰的地点，这些结果表明，种子对交替温度、养分浓度和光照的反应可能是影响该物种种子萌发及其扩散的决定性因素。
