• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

低毒力菌株使油菜植株免疫以抵抗高毒力的黑胫病菌。

Less Virulent Immunizes the Canola Plant to Resist Highly Virulent the Blackleg Pathogen.

作者信息

Padmathilake Kaluhannadige Rasanie Eranka, Fernando Wannakuwattewaduge Gerard Dilantha

机构信息

Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

出版信息

Plants (Basel). 2022 Apr 6;11(7):996. doi: 10.3390/plants11070996.

DOI:10.3390/plants11070996
PMID:35406977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9002471/
Abstract

is a less virulent spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent can boost the resistance of canola plants against the highly virulent . The objective of this study was to confirm the effectiveness of as a biocontrol agent against utilizing morphology, fluorescence microscopy, gene quantification, and transcriptomic analysis. The in planta development of two species inoculated at different time points was assessed using fluorescent protein-tagged isolates which are GFP-tagged and DsRed-tagged . The growth inhibition of by pre-and co-inoculated was supported by no lesion development on cotyledons and no or weak fluorescence protein-tagged mycelia under the confocal microscope. The host defense-related genes, , , , and were upregulated in inoculated Westar cotyledons compared to inoculated cotyledons. The quantification of each pathogen through qPCR assay and gene expressions analysis on host defense-related genes by RT-qPCR confirmed the potential of "brassicae' in the management of the blackleg disease pathogen, 'brassicae', in canola.

摘要

是一种毒性较低的物种，可导致油菜黑胫病。我们实验室之前的研究表明，接种毒性较低的菌株可以增强油菜植株对高毒性菌株的抗性。本研究的目的是利用形态学、荧光显微镜、基因定量和转录组分析来确认作为一种生物防治剂对的有效性。使用绿色荧光蛋白标记的菌株（GFP标记的和DsRed标记的）评估了在不同时间点接种的两种物种在植物体内的发育情况。共聚焦显微镜下，子叶上无病斑形成，荧光蛋白标记的菌丝体无荧光或荧光较弱，这支持了预先接种和共同接种对的生长抑制作用。与接种的子叶相比，接种Westar子叶中与宿主防御相关的基因、、、和上调。通过qPCR测定对每种病原体进行定量，并通过RT-qPCR对宿主防御相关基因进行基因表达分析，证实了“芸苔属”在油菜黑胫病病原体“芸苔属”管理中的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/a29dba6d9242/plants-11-00996-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/f23537edcb71/plants-11-00996-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/f667daa92b06/plants-11-00996-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/b19927bd84fa/plants-11-00996-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/73efe93544e6/plants-11-00996-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/5ad3c7ea7e67/plants-11-00996-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/a29dba6d9242/plants-11-00996-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/f23537edcb71/plants-11-00996-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/f667daa92b06/plants-11-00996-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/b19927bd84fa/plants-11-00996-g003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/73efe93544e6/plants-11-00996-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/5ad3c7ea7e67/plants-11-00996-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c3/9002471/a29dba6d9242/plants-11-00996-g006.jpg

相似文献

1
Less Virulent Immunizes the Canola Plant to Resist Highly Virulent the Blackleg Pathogen.低毒力菌株使油菜植株免疫以抵抗高毒力的黑胫病菌。
Plants (Basel). 2022 Apr 6;11(7):996. doi: 10.3390/plants11070996.
2
Induced Resistance to Blackleg (Leptosphaeria maculans) Disease of Canola (Brassica napus) Caused by a Weakly Virulent Isolate of Leptosphaeria biglobosa.由大孢小球腔菌的弱毒分离株引起的油菜（甘蓝型油菜）对黑胫病（大孢链格孢菌）的诱导抗性
Plant Dis. 2006 Aug;90(8):1059-1064. doi: 10.1094/PD-90-1059.
3
First Report of Leptosphaeria biglobosa 'brassicae' Causing Blackleg on Brassica juncea var. multisecta in China.在中国，大孢小球腔菌‘芸苔变种’引起芥菜型油菜多裂叶变种黑胫病的首次报道
Plant Dis. 2021 May 17. doi: 10.1094/PDIS-10-20-2173-PDN.
4
Genomes and transcriptomes of partners in plant-fungal-interactions between canola (Brassica napus) and two Leptosphaeria species.油菜（甘蓝型油菜）与两种小球腔菌属物种之间植物-真菌相互作用中伙伴的基因组和转录组
PLoS One. 2014 Jul 28;9(7):e103098. doi: 10.1371/journal.pone.0103098. eCollection 2014.
5
First report of Leptosphaeria biglobosa 'canadensis' causing blackleg on oilseed rape (Brassica napus) in China.大孢小球腔菌“加拿大变种”引起中国油菜（甘蓝型油菜）黑胫病的首次报道
Plant Dis. 2021 Mar 23. doi: 10.1094/PDIS-12-20-2735-PDN.
6
First Report of Leptosphaeria biglobosa as a Stem Canker Pathogen of Brassicas in New Zealand.新西兰首次报道大孢小球腔菌为十字花科植物茎溃疡病菌
Plant Dis. 2013 Aug;97(8):1113. doi: 10.1094/PDIS-12-12-1122-PDN.
7
Development of a Duplex qPCR System for Detection and Quantification of the Two Canola Blackleg Pathogens and .建立用于检测和定量两种油菜黑胫病菌和 的双重 qPCR 系统。
Plant Dis. 2023 Sep;107(9):2808-2815. doi: 10.1094/PDIS-10-22-2308-RE. Epub 2023 Sep 20.
8
Detection of Leptosphaeria maculans and Leptosphaeria biglobosa Causing Blackleg Disease in Canola from Canadian Canola Seed Lots and Dockage.检测来自加拿大油菜籽批次及杂质中的、引发油菜黑胫病的黄斑小球腔菌和双球小球腔菌。
Plants (Basel). 2016 Mar 1;5(1):12. doi: 10.3390/plants5010012.
9
First Report on the Presence of Phoma Blackleg Pathogenicity Group 1 (Leptosphaeria biglobosa) on Brassica napus (Canola/Rapeseed) in Iran.关于伊朗甘蓝型油菜（油菜籽）上存在茎点霉黑胫病致病菌群1（大孢小球腔菌）的首次报告。
Plant Dis. 2007 Apr;91(4):465. doi: 10.1094/PDIS-91-4-0465A.
10
First Report of Blackleg Caused by Leptosphaeria maculans on Canola in Idaho.爱达荷州油菜上由黄斑小球腔菌引起的黑胫病的首次报道。
Plant Dis. 2013 Jun;97(6):842. doi: 10.1094/PDIS-10-12-0956-PDN.

引用本文的文献

1
Differences in the Production of Extracellular Polymeric Substances (EPS) and Other Metabolites of () Infecting Winter Oilseed Rape ( L.).侵染冬油菜（甘蓝型油菜）的（某种菌）胞外聚合物（EPS）及其他代谢产物产生的差异 。 （括号内容原文缺失具体信息）
Metabolites. 2023 Jun 17;13(6):759. doi: 10.3390/metabo13060759.

本文引用的文献

1
Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the - Pathosystem.在 - 植物病原互作体系中，对易感性、中间性和抗性互作的激素响应。
Int J Mol Sci. 2021 Apr 29;22(9):4714. doi: 10.3390/ijms22094714.
2
Presence of the Weakly Pathogenic in the Fusarium Head Blight Disease Complex Hampers Biocontrol and Chemical Control of the Virulent Pathogen.镰刀菌穗腐病复合病害中弱致病性病原菌的存在阻碍了对强致病性病原菌的生物防治和化学防治。
Front Plant Sci. 2021 Feb 17;12:641890. doi: 10.3389/fpls.2021.641890. eCollection 2021.
3
Protection to Tomato Wilt Disease Conferred by the Nonpathogen Fo47 is More Effective Than that Conferred by Avirulent Strains.
非致病菌 Fo47 对番茄黄萎病的保护作用强于无毒菌株。
Phytopathology. 2021 Feb;111(2):253-257. doi: 10.1094/PHYTO-04-20-0133-R. Epub 2020 Nov 21.
4
The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato-Potato Virus Y (PVY) Hypersensitive and Susceptible Reactions.马铃薯-马铃薯 Y 病毒（PVY）感病和抗病反应中呼吸爆发氧化酶同源物 D（RbohD）的细胞和组织分布。
Int J Mol Sci. 2019 Jun 4;20(11):2741. doi: 10.3390/ijms20112741.
5
A New Subclade of Identified from .从 中鉴定出的一个新的亚分支。
Int J Mol Sci. 2019 Apr 3;20(7):1668. doi: 10.3390/ijms20071668.
6
Transcriptome Analysis of -Mediated Host Immunity in the - Pathosystem.转录组分析在 - 致病系统中 - 介导的宿主免疫。
Mol Plant Microbe Interact. 2019 Aug;32(8):1001-1012. doi: 10.1094/MPMI-01-19-0028-R. Epub 2019 Jun 25.
7
Induced Resistance to Blackleg (Leptosphaeria maculans) Disease of Canola (Brassica napus) Caused by a Weakly Virulent Isolate of Leptosphaeria biglobosa.由大孢小球腔菌的弱毒分离株引起的油菜（甘蓝型油菜）对黑胫病（大孢链格孢菌）的诱导抗性
Plant Dis. 2006 Aug;90(8):1059-1064. doi: 10.1094/PD-90-1059.
8
Signaling Crosstalk between Salicylic Acid and Ethylene/Jasmonate in Plant Defense: Do We Understand What They Are Whispering?水杨酸和乙烯/茉莉酸信号交叉对话在植物防御中的作用：我们是否理解它们在说什么？
Int J Mol Sci. 2019 Feb 4;20(3):671. doi: 10.3390/ijms20030671.
9
Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes.创伤通过动态激素和转录变化引发骨痂形成。
Plant Physiol. 2017 Nov;175(3):1158-1174. doi: 10.1104/pp.17.01035. Epub 2017 Sep 13.
10
Transcriptome analysis of the Brassica napus-Leptosphaeria maculans pathosystem identifies receptor, signaling and structural genes underlying plant resistance.油菜-茎点霉互作转录组分析鉴定了植物抗性相关的受体、信号和结构基因。
Plant J. 2017 May;90(3):573-586. doi: 10.1111/tpj.13514. Epub 2017 Mar 27.