Less Virulent Immunizes the Canola Plant to Resist Highly Virulent the Blackleg Pathogen.

is a less virulent spp. that causes blackleg disease in canola. Previous studies from our lab have shown that inoculation with the less virulent can boost the resistance of canola plants against the highly virulent . The objective of this study was to confirm the effectiveness of as a biocontrol agent against utilizing morphology, fluorescence microscopy, gene quantification, and transcriptomic analysis. The in planta development of two species inoculated at different time points was assessed using fluorescent protein-tagged isolates which are GFP-tagged and DsRed-tagged . The growth inhibition of by pre-and co-inoculated was supported by no lesion development on cotyledons and no or weak fluorescence protein-tagged mycelia under the confocal microscope. The host defense-related genes, , , , and were upregulated in inoculated Westar cotyledons compared to inoculated cotyledons. The quantification of each pathogen through qPCR assay and gene expressions analysis on host defense-related genes by RT-qPCR confirmed the potential of "brassicae' in the management of the blackleg disease pathogen, 'brassicae', in canola.