Satjarak Anchittha, Graham Linda E, Trest Marie T, Arancibia-Avila Patricia
Plants of Thailand Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Botany, University of Wisconsin, Madison, WI 53706-1313, USA.
Plants (Basel). 2022 Apr 6;11(7):1001. doi: 10.3390/plants11071001.
The modern pteridophyte genus is the only survivor of Sphenopsida, an ancient clade known from the Devonian. This genus, of nearly worldwide distribution, comprises approximately 15 extant species. However, genomic information is limited. In this study, we assembled the complete chloroplast genome of the giant species from a metagenomic sequence and compared the plastid genome structure and protein-coding regions with information available for two other species using network analysis. chloroplast genomes showed conserved traits of quadripartite structure, gene content, and gene order. Phylogenetic analysis based on plastome protein-coding regions corroborated previous reports that is monophyletic, and that is more closely related to than to . Single-gene phylogenetic estimation and haplotype analysis showed that belonged to the subgenus . Single-gene haplotype analysis revealed that , , , and resolved more than one haplotype per species, suggesting the presence of a high diversity or a high mutation rate of the corresponding nucleotide sequence. Sequences from appeared as a distinct group of haplotypes representing the subgenus that diverged from and . In addition, the taxa that were frequently located at the joint region of the map were and , suggesting the presence of some plastome characters among the subgenera.
现代蕨类植物属是楔叶纲唯一的幸存者，楔叶纲是一个可追溯到泥盆纪的古老分支。该属分布几乎遍及全球，现存约15个物种。然而，其基因组信息有限。在本研究中，我们从宏基因组序列中组装了巨型物种的完整叶绿体基因组，并使用网络分析将质体基因组结构和蛋白质编码区域与另外两个物种的可用信息进行了比较。叶绿体基因组显示出四分体结构、基因含量和基因顺序的保守特征。基于质体基因组蛋白质编码区域的系统发育分析证实了先前的报道，即该属是单系的，并且与该属相比，该属与该属的关系更为密切。单基因系统发育估计和单倍型分析表明，该属属于亚属。单基因单倍型分析表明，该属每个物种都解析出不止一种单倍型，这表明相应核苷酸序列存在高多样性或高突变率。该属的序列表现为代表亚属的一组独特单倍型，与该属和该属不同。此外，在图谱联合区域经常出现的分类群是该属和该属，这表明该亚属之间存在一些质体基因组特征。
