Du Han, Ping Tao, Wu Wei, Yang Qingli
College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
Foods. 2022 Mar 25;11(7):945. doi: 10.3390/foods11070945.
Timely detection of () is critical because it can multiply to disease-causing levels in a matter of hours. Herein, a simple and sensitive DNA tetrahedral (Td) fluorescence signal amplifier with blue carbon quantum dots (bCDs) was prepared for sandwich detection of . bCD was modified at the apex of Td, and an aptamer on Td was used to accurately identify and "adsorb" the amplifier to the surface of . Atomic force microscopy (AFM) demonstrates the successful preparation of this signal amplifier. The fluorescence intensity emitted in this strategy increased 4.72 times. The strategy showed a stronger fluorescence intensity change, sensitivity (linear range of 7.22 × 10-1.44 × 10 CFU/mL with a LOD of 4 CFU/mL), and selectivity. The recovery rate in qualified pasteurized milk and drinking water samples was 96.54% to 104.72%. Compared with simple aptamer sandwich detection, these fluorescence signal amplifiers have improved fluorescence detection of . Additionally, this fluorescent signal amplification strategy may be applied to the detection of other food pathogens or environmental microorganisms in the future.
及时检测（）至关重要，因为它能在数小时内繁殖到致病水平。在此，制备了一种带有蓝色碳量子点（bCDs）的简单且灵敏的DNA四面体（Td）荧光信号放大器，用于夹心检测（）。bCD修饰在Td的顶点，Td上的适配体用于准确识别并将放大器“吸附”到（）表面。原子力显微镜（AFM）证明了这种信号放大器的成功制备。该策略发出的荧光强度增加了4.72倍。该策略显示出更强的荧光强度变化、灵敏度（线性范围为7.22×10 - 1.44×10 CFU/mL，检测限为4 CFU/mL）和选择性。合格巴氏杀菌牛奶和饮用水样品中的回收率为96.54%至104.72%。与简单的适配体夹心检测相比，这些荧光信号放大器改善了对（）的荧光检测。此外，这种荧光信号放大策略未来可能应用于其他食品病原体或环境微生物的检测。
