Gao Xue, Zhang Hongmei, Liu Lu, Jia Mu, Li Xuepeng, Li Jianrong
College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Jinzhou, Liaoning 121013, China.
College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Jinzhou, Liaoning 121013, China.
Food Chem. 2024 Jan 30;432:137144. doi: 10.1016/j.foodchem.2023.137144. Epub 2023 Aug 15.
A ratiometric fluorescence and colorimetry dual-mode nano-biosensor has been established for Staphylococcus aureus (S. aureus) determination. The prepared approaches of Manganese dioxide nanosheets (MnO NSs) and carbon dots (BCDs) were facile, efficient and labor-saving and MnO NSs-mediated fluorescence quenching and oxidation could amplify detection signals. The dual-mode determination had a broad linear range of 37 ∼ 3.7 × 10 CFU/mL and low detection limits of 9 CFU/mL (ratiometric fluorescence) and 22 CFU/mL (colorimetry). Meanwhile, the method was applied in real samples with recovery ranging of 90 ∼ 102% and RSD < 4.44%, which was an insignificant difference with standard plate counting. The new dual-mode approach of S. aureus possesses the advantages of superior sensitivity, precision, accuracy and specificity. Moreover, the dual-mode nano-biosensor can be adopted in other foodborne pathogens determination by changing corresponding aptamers and provide an enlightenment in monitoring food safety.
已建立一种用于金黄色葡萄球菌（S. aureus）测定的比率荧光和比色法双模式纳米生物传感器。二氧化锰纳米片（MnO NSs）和碳点（BCDs）的制备方法简便、高效且省力，MnO NSs介导的荧光猝灭和氧化可放大检测信号。双模式测定的线性范围宽，为37 ∼ 3.7×10 CFU/mL，检测限低，比率荧光法为9 CFU/mL，比色法为22 CFU/mL。同时，该方法应用于实际样品，回收率范围为90 ∼ 102%，相对标准偏差<4.44%，与标准平板计数法无显著差异。金黄色葡萄球菌的新双模式方法具有灵敏度高、精密度高、准确度高和特异性强的优点。此外，通过更换相应的适配体，双模式纳米生物传感器可用于其他食源性病原体的测定，为食品安全监测提供了启示。
