Coelho Marta, Oliveira Carla, Coscueta Ezequiel R, Fernandes João, Pereira Ricardo N, Teixeira José A, Rodrigues António Sebastião, Pintado Manuela E
CBQF-Centro de Biotecnologia e Química Fina-Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.
LABBELS-Associate Laboratory-CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Foods. 2022 Apr 6;11(7):1064. doi: 10.3390/foods11071064.
A nutrient-rich diet is a key to improving the chemical signals, such as antioxidants, which modulate pathogens' resistance in the gut and prevent diseases. A current industrial problem is the generation of undervalued by-products, such as tomato bagasse, which are rich in bioactive compounds and of commercial interest (carotenoids and phenolic compounds). This work analyzed the effect of gastrointestinal digestion on the bioactivity and bioaccessibility of carotenoids and phenolic compounds from tomato bagasse extracts. Thus, the extraction by ohmic heating (OH) technology was compared with conventional (organic solvents). The results showed that the main phenolic compounds identified by UPLC-qTOF-MS were p-coumaric acid, naringenin, and luteolin. A higher recovery index for total phenolic compounds throughout the gastrointestinal digestion was observed for OH while for carotenoids, a strong reduction after stomach conditions was observed for both extracts. Furthermore, colon-available fraction exhibited a prebiotic effect upon different and , but a strain-dependent and more accentuated effect on OH. Thus, the extraction technology highly influenced bioaccessibility, with OH demonstrating a positive impact on the recovery of bioactive compounds and related health benefits, such as antioxidant, anti-hypertensive, prebiotic, and anti-inflammatory properties. Of these properties, the last is demonstrated here for the first time.
营养丰富的饮食是改善化学信号（如抗氧化剂）的关键，这些化学信号可调节肠道中病原体的抵抗力并预防疾病。当前的一个工业问题是产生了价值被低估的副产品，例如番茄渣，其富含生物活性化合物且具有商业价值（类胡萝卜素和酚类化合物）。这项工作分析了胃肠道消化对番茄渣提取物中类胡萝卜素和酚类化合物的生物活性及生物可及性的影响。因此，将欧姆加热（OH）技术的提取效果与传统方法（有机溶剂）进行了比较。结果表明，通过超高效液相色谱-四极杆飞行时间质谱（UPLC-qTOF-MS）鉴定出的主要酚类化合物为对香豆酸、柚皮素和木犀草素。在胃肠道消化过程中，观察到OH法提取的总酚类化合物的回收率更高，而对于类胡萝卜素，两种提取物在胃部消化条件后均出现了大幅减少。此外，结肠可利用部分对不同的[此处原文缺失相关内容]表现出益生元效应，但对OH法有菌株依赖性且影响更为明显。因此，提取技术对生物可及性有很大影响，OH法对生物活性化合物的回收率以及相关的健康益处（如抗氧化、抗高血压、益生元和抗炎特性）具有积极影响。其中，抗炎特性在此首次得到证实。
