Al-Khafaji Mohammed A, Gaál Anikó, Jezsó Bálint, Mihály Judith, Varga Zoltán
Research Centre for Natural Sciences, Institute of Materials and Environmental Chemistry, H-1117 Budapest, Hungary.
Hevesy György PhD School of Chemistry, Eötvös Loránd University, H-1117 Budapest, Hungary.
Materials (Basel). 2022 Apr 6;15(7):2696. doi: 10.3390/ma15072696.
Surface modification of silica nanoparticles with organic functional groups while maintaining colloidal stability remains a synthetic challenge. This work aimed to prepare highly dispersed porous hollow organosilica particles (pHOPs) with amino surface modification. The amino-surface modification of pHOPs was carried out with 3-aminopropyl(diethoxy)methylsilane (APDEMS) under various reaction parameters, and the optimal pHOP-NH sample was selected and labelled with fluorescein isothiocyanate (FITC) to achieve fluorescent pHOPs (F-HOPs). The prepared pHOPs were thoroughly characterized by transmission electron microscopy, dynamic light scattering, FT-IR, UV-Vis and fluorescence spectroscopies, and microfluidic resistive pulse sensing. The optimal amino surface modification of pHOPs with APDEMS was at pH 10.2, at 60 °C temperature with 10 min reaction time. The positive Zeta potential of pHOP-NH in an acidic environment and the appearance of vibrations characteristic to the surface amino groups on the FT-IR spectra prove the successful surface modification. A red-shift in the absorbance spectrum and the appearance of bands characteristic to secondary amines in the FTIR spectrum of F-HOP confirmed the covalent attachment of FITC to pHOP-NH. This study provides a step-by-step synthetic optimization and characterization of fluorescently labelled organosilica particles to enhance their optical properties and extend their applications.
在保持胶体稳定性的同时，用有机官能团对二氧化硅纳米颗粒进行表面改性仍然是一个合成挑战。这项工作旨在制备具有氨基表面改性的高度分散的多孔中空有机硅颗粒（pHOPs）。在各种反应参数下，用3-氨丙基（二乙氧基）甲基硅烷（APDEMS）对pHOPs进行氨基表面改性，并选择最佳的pHOP-NH样品并用异硫氰酸荧光素（FITC）标记，以获得荧光pHOPs（F-HOPs）。通过透射电子显微镜、动态光散射、傅里叶变换红外光谱、紫外可见光谱和荧光光谱以及微流控电阻脉冲传感对制备的pHOPs进行了全面表征。用APDEMS对pHOPs进行最佳氨基表面改性的条件是pH值为10.2，温度为60°C，反应时间为10分钟。pHOP-NH在酸性环境中的正Zeta电位以及傅里叶变换红外光谱上表面氨基特征振动的出现证明了表面改性的成功。F-HOP的吸收光谱中的红移以及傅里叶变换红外光谱中仲胺特征谱带的出现证实了FITC与pHOP-NH的共价连接。本研究提供了荧光标记有机硅颗粒的逐步合成优化和表征，以增强其光学性能并扩展其应用。
