Biomolecular Research Centre, Sheffield Hallam University, Sheffield S1 1WB, UK.
Bruker Daltonics GmbH & Co. KG, 28359 Bremen, Germany.
Molecules. 2022 Mar 22;27(7):2039. doi: 10.3390/molecules27072039.
Recently published work has reported the development and application of a bottom-up proteomic approach to distinguish between human and animal blood (down to animal species level), by rapid screening using Matrix Assisted Laser Desorption Ionisation Mass Spectrometry (MALDI MS). In that study, it was additionally observed that intravenous animal blood exhibits different spectral profiles from blood collected within the animal chest cavity as well as from the diluted blood collected within packets of meat. In this follow-up study we explored the resulting hypothesis that, depending on how blood is shed or collected, protein biomarker profiles vary to the extent of systematically permitting a distinction between possible sources of blood (for example, flesh wound versus packaged meat). This intelligence may be important in reconstructing the dynamics of the crime. The combination of statistical analysis and tandem mass spectrometry has yielded additional animal blood markers as well as confirming the ability to correctly determine the animal species from which blood derived, regardless of the retailer selling it (amongst the five investigated). These data confirm the initial hypothesis and demonstrate the opportunity for the proteomics-MALDI combined approach to provide additional intelligence to the investigation of violent crimes when examining blood evidence.
最近发表的工作报告了一种自下而上的蛋白质组学方法的发展和应用，该方法通过基质辅助激光解吸电离质谱（MALDI MS）的快速筛选，可以区分人和动物的血液（低至动物物种水平）。在该研究中，还观察到静脉内动物血液的光谱特征与从动物胸腔内采集的血液以及从肉包装中采集的稀释血液不同。在这项后续研究中，我们探讨了由此产生的假设，即根据血液的流失或采集方式，蛋白质生物标志物的特征会发生变化，以至于可以系统地区分可能的血液来源（例如，肉体伤口与包装肉）。这种情报可能对重建犯罪动态很重要。统计分析和串联质谱的结合产生了额外的动物血液标志物，并证实了无论零售商如何销售（在五个调查对象中），都能够正确确定血液来源的动物种类。这些数据证实了最初的假设，并表明蛋白质组学-MALDI 联合方法有机会在检查血液证据时为暴力犯罪调查提供额外的情报。
