Bradshaw R, Bleay S, Clench M R, Francese S
Biomedical Research Centre, Sheffield Hallam University, Howard Street, S1 1WB Sheffield, UK.
Center for Applied Science and Technologies, Home Office, St Albans, Hertfordshire AL4 9HQ, UK.
Sci Justice. 2014 Mar;54(2):110-7. doi: 10.1016/j.scijus.2013.12.004. Epub 2014 Jan 3.
The determination of the presence of blood in fingermarks constitutes important intelligence in a criminal investigation as it helps to reconstruct the events that have taken place at a scene of crime. Various methodologies have been reported and are currently employed for the detection of the presence of blood including optical, spectroscopic and chemical development approaches. However, most methods only give an indication that blood may be present and, therefore, these methods are described as presumptive tests. Here we show the use of Matrix-Assisted Laser Desorption Ionisation Mass Spectrometry Profiling and Imaging (MALDI MSP and MALDI MSI) for the determination of the presence of blood in fingermarks by specifically detecting the molecules of haem and haemoglobin through their mass-to-charge ratios. Furthermore, preliminary experiments are shown which demonstrate that this technology is compatible with other methods currently employed for enhancing fingermarks in blood (or contaminated by blood). The application of the developed protocols to a crime scene blood trace, demonstrates the feasibility of using this technology in routine casework. These findings open up a new line of research for the development of robust MALDI MSP and MALDI MSI protocols for the detection and chemical imaging of bloodied marks.
确定指纹中是否存在血液在刑事调查中构成重要线索，因为它有助于重现犯罪现场发生的事件。已经报道了各种方法，目前也在使用多种方法来检测血液的存在，包括光学、光谱和化学显影方法。然而，大多数方法只能表明可能存在血液，因此，这些方法被称为推定测试。在此，我们展示了使用基质辅助激光解吸电离质谱分析和成像（MALDI MSP和MALDI MSI）通过特异性检测血红素和血红蛋白分子的质荷比来确定指纹中是否存在血液。此外，还展示了初步实验，证明该技术与目前用于增强血液中（或被血液污染）指纹的其他方法兼容。将所开发的方案应用于犯罪现场血迹痕迹，证明了在常规案件工作中使用该技术的可行性。这些发现为开发用于检测和化学成像带血痕迹的强大MALDI MSP和MALDI MSI方案开辟了一条新的研究路线。
